Evidence map›Paper›PMID 34391819›Full record

ArticleJournal of microbiological methods2021

An adaptable and non-invasive method for tracking Bifidobacterium animalis subspecies lactis 420 in the mouse gut.

Marissa A Lopez-Pier, Matthew P Koppinger, Preston R Harris, Danielle K Cannon, Rinku S Skaria, Bonnie L Hurwitz, George Watts, Shravan Aras, Marvin J Slepian, John P Konhilas

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of microbiological methods, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.2field-weighted citation impact, top 47% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 1 institution in 1 country.

Marissa A Lopez-PierSarver Molecular Cardiovascular Research Program, University of Arizona, Tucson, AZ, USA; Department of Biomedical Engineering, University of Arizona, Tucson, AZ, USA.
Matthew P KoppingerSarver Molecular Cardiovascular Research Program, University of Arizona, Tucson, AZ, USA; Department of Nutritional Sciences, University of Arizona, Tucson, AZ, USA.
Preston R HarrisSarver Molecular Cardiovascular Research Program, University of Arizona, Tucson, AZ, USA; Department of Nutritional Sciences, University of Arizona, Tucson, AZ, USA.
Danielle K CannonDepartment of Physiology, University of Arizona, Tucson, AZ, USA; Sarver Molecular Cardiovascular Research Program, University of Arizona, Tucson, AZ, USA.
Rinku S SkariaDepartment of Physiology, University of Arizona, Tucson, AZ, USA; Sarver Molecular Cardiovascular Research Program, University of Arizona, Tucson, AZ, USA.
Bonnie L HurwitzBio5 Institute, University of Arizona, Tucson, AZ, USA.
George WattsBio5 Institute, University of Arizona, Tucson, AZ, USA.
Shravan ArasMenolabs, LLC, Tucson, AZ, USA.
Marvin J SlepianSarver Molecular Cardiovascular Research Program, University of Arizona, Tucson, AZ, USA; Department of Biomedical Engineering, University of Arizona, Tucson, AZ, USA; Bio5 Institute, University of Arizona, Tucson, AZ, USA; Department of Medicine, University of Arizona, Tucson, AZ, USA.
John P KonhilasDepartment of Physiology, University of Arizona, Tucson, AZ, USA; Sarver Molecular Cardiovascular Research Program, University of Arizona, Tucson, AZ, USA; Department of Biomedical Engineering, University of Arizona, Tucson, AZ, USA; Department of Molecular and Cellular Biology, University of Arizona, Tucson, AZ, USA; Department of Cellular and Molecular Medicine, University of Arizona, Tucson, AZ, USA; Department of Nutritional Sciences, University of Arizona, Tucson, AZ, USA. Electronic address: konhilas@email.arizona.edu.
University of Arizona · US

Funding

SOUTHWEST ENVIRONMENTAL HEALTH SCIENCES CENTERP30ES006694 · NIEHS · UNIVERSITY OF ARIZONA · PI DEAN BILLHEIMER · 1994 to 2026
$36.7M
PHYSIOLOGYT32HL007249 · NHLBI · UNIVERSITY OF ARIZONA · PI Brett A Colson, JOHN P KONHILAS · 1985 to 2026
$12.5M
Computer Model of the Canine VentricleR01HL075515 · NHLBI · CORNELL UNIVERSITY ITHACA · PI GILMOUR, ROBERT F · 2004 to 2009
$2.5M
Computational and Mathematical Modeling of Biomedical SystemsT32GM132008 · NIGMS · UNIVERSITY OF ARIZONA · PI Ryan Gutenkunst, JOANNA MASEL · 2019 to 2026
$2.3M
Impact of AMP-activated kinase on sex differences in hypertrophic cardiomyopathyR01HL098256 · NHLBI · UNIVERSITY OF ARIZONA · PI KONHILAS, JOHN P · 2010 to 2014
$1.9M
NHLBI NIH HHS R01 HL075515NHLBI NIH HHS R01 HL098256NHLBI NIH HHS T32 HL007249NIEHS NIH HHS P30 ES006694NIGMS NIH HHS T32 GM132008
6 · The paper itself

Abstract

Probiotic strains from the Bifidobacterium or Lactobacillus genera improve health outcomes in models of metabolic and cardiovascular disease. Yet, underlying mechanisms governing these improved health outcomes are rooted in the interaction of gut microbiota, intestinal interface, and probiotic strain. Central to defining the underlying mechanisms governing these improved health outcomes is the development of adaptable and non-invasive tools to study probiotic localization and colonization within the host gut microbiome. The objective of this study was to test labeling and tracking efficacy of Bifidobacterium animalis subspecies lactis 420 (B420) using a common clinical imaging agent, indocyanine green (ICG). ICG was an effective in situ labeling agent visualized in either intact mouse or excised gastrointestinal (GI) tract at different time intervals. Quantitative PCR was used to validate ICG visualization of B420, which also demonstrated that B420 transit time matched normal murine GI motility (~8 hours). Contrary to previous thoughts, B420 did not colonize any region of the GI tract whether following a single bolus or daily administration for up to 10 days. We conclude that ICG may provide a useful tool to visualize and track probiotic species such as B420 without implementing complex molecular and genetic tools. Proof-of-concept studies indicate that B420 did not colonize and establish residency align the murine GI tract.

Indexed as

Gastrointestinal MicrobiomeAnimalsBacterial TranslocationBifidobacterium animalisGastrointestinal TractIndocyanine GreenMaleMiceMice, Inbred C57BLOptical ImagingProbioticsStaining and LabelingIndocyanine GreenBifidobacterium animalis lactis subspecies 420Indocyanine greenISOVUE-300Quantitative PCRTranslocation

Identifiers

PMID34391819
PMCPMC8473990
OpenAlexW3196105668

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.