ArticleVirology journal2021
First molecular characterization of poxviruses in cattle, sheep, and goats in Botswana.
Article in Virology journal, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 17 citations in OpenAlex.
- Genetic Characterisation and Risk Factors Associated With Capripoxvirus Outbreaks Among Small Ruminants in Uganda, 2021-2022.Veterinary medicine and science · 2026Article
- A novel ORF127/ORF130 gene deletion Orf virus vaccine candidate: construction and evaluation.Virology journal · 2026Article
- First outbreak of lumpy skin disease in Japan, 2024.The Journal of veterinary medical science · 2025Article
- Lumpy Skin Disease Virus (LSDV): evaluating global impact, Ethiopia's response strategies, and the promise of gamma-ray inactivated vaccines.Tropical animal health and production · 2025Review
- Progress in diagnostic methods and vaccines for lumpy skin disease virus: a path towards understanding the disease.Veterinary research communications · 2025Review
- Lumpy skin disease: A growing threat to the global livestock industry.Open veterinary journal · 2025Review
- Seroprevalence and risk factors for sheep pox and goat pox among small ruminant herds from Nakapiripirit and Sembabule districts, Uganda.Frontiers in veterinary science · 2025Article
- Detection and molecular characterization of lumpy skin disease and bovine papular stomatitis viruses in lumpy skin disease-suspected outbreaks in Tanzania.Virology journal · 2024Article
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- Review
- Article
- Clinicopathological and molecular studies on cattle naturally infected with lumpy skin diseases in selected districts of Wolaita Zone, Southern Ethiopia.BMC veterinary research · 2022Article
- Understanding the research advances on lumpy skin disease: A comprehensive literature review of experimental evidence.Frontiers in microbiology · 2022Review
- A survey of contagious ecthyma and molecular characterization of Orf virus in sheep and goats in Nigeria (2014-2016).Open veterinary journalArticle
Corrections and comments
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Authors and funding
14 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPoxviruses within the Capripoxvirus, Orthopoxvirus, and Parapoxvirus genera can infect livestock, with the two former having zoonotic importance. In addition, they induce similar clinical symptoms in common host species, creating a challenge for diagnosis. Although endemic in the country, poxvirus infections of small ruminants and cattle have received little attention in Botswana, with no prior use of molecular tools to diagnose and characterize the pathogens.
methodsA high-resolution melting (HRM) assay was used to detect and differentiate poxviruses in skin biopsy and skin scab samples from four cattle, one sheep, and one goat. Molecular characterization of capripoxviruses and parapoxviruses was undertaken by sequence analysis of RPO30 and GPCR genes.
resultsThe HRM assay revealed lumpy skin disease virus (LSDV) in three cattle samples, pseudocowpox virus (PCPV) in one cattle sample, and orf virus (ORFV) in one goat and one sheep sample. The phylogenetic analyses, based on the RPO30 and GPCR multiple sequence alignments showed that the LSDV sequences of Botswana were similar to common LSDV field isolates encountered in Africa, Asia, and Europe. The Botswana PCPV presented unique features and clustered between camel and cattle PCPV isolates. The Botswana ORFV sequence isolated from goat differed from the ORFV sequence isolated from sheep.
conclusionsThis study is the first report on the genetic characterization of poxvirus diseases circulating in cattle, goats, and sheep in Botswana. It shows the importance of molecular methods to differentially diagnose poxvirus diseases of ruminants.
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