ArticleJournal of neuroscience methods2021
Methods for intratumoral microdialysis probe targeting and validation in murine brain tumor models.
Article in Journal of neuroscience methods, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed, 5 citations in OpenAlex.
- Article
- Exploring the Application of the Artificial-Intelligence-Integrated Platform 3D Slicer in Medical Imaging Education.Diagnostics (Basel, Switzerland) · 2024Review
- Advances in computational and translational approaches for malignant glioma.Frontiers in physiology · 2023Review
- Parametric study of a microdialysis probe and study of depletion effect using ethanol as a test analyte.Biochemical and biophysical research communications · 2022Article
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Authors and funding
13 authors at 3 institutions in 1 country.
Funding
Abstract
backgroundMicrodialysis is a well validated sampling technique that can be used for pharmacokinetic studies of oncological drugs targeting the central nervous system. This technique has also been applied to evaluate tumor metabolism and identify pharmacodynamic biomarkers of drug activity. Despite the potential utility of microdialysis for therapeutic discovery, variability in tumor size and location hamper routine use of microdialysis as a preclinical tool. Quantitative validation of microdialysis membrane location relative to radiographically evident tumor regions could facilitate rigorous preclinical studies. However, a widely accessible standardized workflow for preclinical catheter placement and validation is needed. NEW
methodWe provide methods for a workflow to yield tailored placement of microdialysis probes within a murine intracranial tumor and illustrate in an IDH1-mutant patient-derived xenograft (PDX) model. This detailed workflow uses a freely available on-line tool built within 3D-slicer freeware to target microdialysis probe placement within the tumor core and validate probe placement fully within the tumor.
resultsWe illustrate use of this workflow to validate microdialysis probe location relative to implanted IDH1-mutant PDXs, using the microdialysis probes to quantify levels of extracellular onco-metabolite D-2 hydroxyglutarate. COMPARISON WITH EXISTING
methodsPrevious methods have used 3D slicer to reliably measure tumor volumes. Prior microdialysis studies have targeted expected tumor locations without validation.
conclusionsThe new method offers a streamlined and freely available workflow in 3D slicer to optimize and validate microdialysis probe placement within a murine brain tumor.
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