Evidence map›Paper›PMID 34390064›Full record

ArticleCell proliferation2021

The combination of dextran sulphate and polyvinyl alcohol prevents excess aggregation and promotes proliferation of pluripotent stem cells in suspension culture.

Xianglian Tang, Haibin Wu, Jinghe Xie, Ning Wang, Qicong Chen, Zhiyong Zhong, Yaqi Qiu, Jue Wang, Xiajing Li, Ping Situ and 4 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell proliferation, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
0.9field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 15 citations in OpenAlex.

  1. Stem cell therapies for diabetes.Nature medicine · 2025
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 4 institutions in 2 countries.

Xianglian TangSchool of Biomedical Sciences and Engineering, Guangzhou International Campus, South China University of Technology, Guangzhou, China.ORCID https://orcid.org/0000-0002-4101-4391
Haibin WuLaboratory of Stem Cells and Translational Medicine, Institutes for Life Sciences and School of Medicine, South China University of Technology, Guangzhou, China.
Jinghe XieSchool of Biomedical Sciences and Engineering, Guangzhou International Campus, South China University of Technology, Guangzhou, China.
Ning WangSchool of Biomedical Sciences and Engineering, Guangzhou International Campus, South China University of Technology, Guangzhou, China.
Qicong ChenSchool of Biomedical Sciences and Engineering, Guangzhou International Campus, South China University of Technology, Guangzhou, China.
Zhiyong ZhongSchool of Biomedical Sciences and Engineering, Guangzhou International Campus, South China University of Technology, Guangzhou, China.
Yaqi QiuLaboratory of Stem Cells and Translational Medicine, Institutes for Life Sciences and School of Medicine, South China University of Technology, Guangzhou, China.
Jue WangLaboratory of Stem Cells and Translational Medicine, Institutes for Life Sciences and School of Medicine, South China University of Technology, Guangzhou, China.
Xiajing LiGuangzhou First People's Hospital, School of Medicine, South China University of Technology, Guangzhou, China.
Ping SituLaboratory of Stem Cells and Translational Medicine, Institutes for Life Sciences and School of Medicine, South China University of Technology, Guangzhou, China.
Liangxue LaiKey Laboratory of Regenerative Biology, South China Institute for Stem Cell, Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China.
Mark A ZernDepartment of Internal Medicine, University of California Davis Medical Center, Sacramento, CA, USA.
Honglin ChenLaboratory of Stem Cells and Translational Medicine, Institutes for Life Sciences and School of Medicine, South China University of Technology, Guangzhou, China.
Yuyou DuanLaboratory of Stem Cells and Translational Medicine, Institutes for Life Sciences and School of Medicine, South China University of Technology, Guangzhou, China.ORCID https://orcid.org/0000-0002-1402-7402
Cell Technology (China) · CNChinese Academy of Sciences · CNGuangzhou First People's Hospital · CNUniversity of California Davis Medical Center · US

Funding

National Key Research and Development Program of China 2018YFA0108200National Natural Science Foundation of China 31900976National Natural Science Foundation of China 32071360Research Agreement between South China University of Technology and Guangzhou First People's Hospital D9194290Research Agreement between South China University of Technology and Guangzhou First People's Hospital PT31900976Research Starting Funding of South China University of Technology D6181910Research Starting Funding of South China University of Technology D6201880Research Starting Funding of South China University of Technology K5180910Research Starting Funding of South China University of Technology K5204120
6 · The paper itself

Abstract

objectivesFor clinical applications of cell-based therapies, a large quantity of human pluripotent stem cells (hPSCs) produced in standardized and scalable culture processes is required. Currently, microcarrier-free suspension culture shows potential for large-scale expansion of hPSCs; however, hPSCs tend to aggregate during culturing leading to a negative effect on cell yield. To overcome this problem, we developed a novel protocol to effectively control the sizes of cell aggregates and enhance the cell proliferation during the expansion of hPSCs in suspension. MATERIALS AND

methodshPSCs were expanded in suspension culture supplemented with polyvinyl alcohol (PVA) and dextran sulphate (DS), and 3D suspension culture of hPSCs formed cell aggregates under static or dynamic conditions. The sizes of cell aggregates and the cell proliferation as well as the pluripotency of hPSCs after expansion were assessed using cell counting, size analysis, real-time quantitative polymerase chain reaction, flow cytometry analysis, immunofluorescence staining, embryoid body formation, teratoma formation and transcriptome sequencing.

resultsOur results demonstrated that the addition of DS alone effectively prevented hPSC aggregation, while the addition of PVA significantly enhanced hPSC proliferation. The combination of PVA and DS not only promoted cell proliferation of hPSCs but also produced uniform and size-controlled cell aggregates. Moreover, hPSCs treated with PVA, or DS or a combination, maintained the pluripotency and were capable of differentiating into all three germ layers. mRNA-seq analysis demonstrated that the combination of PVA and DS significantly promoted hPSC proliferation and prevented cell aggregation through improving energy metabolism-related processes, regulating cell growth, cell proliferation and cell division, as well as reducing the adhesion among hPSC aggregates by affecting expression of genes related to cell adhesion.

conclusionsOur results represent a significant step towards developing a simple and robust approach for the expansion of hPSCs in large scale.

Indexed as

AnimalsBioreactorsCell AggregationCell Culture TechniquesCell DifferentiationCell ProliferationCells, CulturedDextran SulfateHumansMicePluripotent Stem CellsPolyvinyl AlcoholDextran SulfatePolyvinyl Alcoholcell aggregatesdextran sulphatehuman pluripotent stem cellspolyvinyl alcoholspinner flasksuspension culture

Identifiers

PMID34390064
PMCPMC8450127
OpenAlexW3189644765

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.