Evidence map›Paper›PMID 34389949›Full record

ArticleEnvironmental science and pollution research international2022

Annotation of chemotaxis gene clusters and proteins involved in chemotaxis of Bacillus subtilis strain MB378 capable of biodecolorizing different dyes.

Uruj Tahir, Fozia Aslam, Shiza Nawaz, Umair Hassan Khan, Azra Yasmin

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Article in Environmental science and pollution research international, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.6field-weighted citation impact, top 35% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 10 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 4 institutions in 2 countries.

Uruj TahirMicrobiology and Biotechnology Laboratory, Department of Environmental Sciences, Fatima Jinnah Women University, Rawalpindi, 46000, Pakistan. urujtahirjavaid@gmail.com.ORCID http://orcid.org/0000-0002-5393-5278
Fozia AslamMicrobiology and Biotechnology Laboratory, Department of Environmental Sciences, Fatima Jinnah Women University, Rawalpindi, 46000, Pakistan.
Shiza NawazNational Institute for Biotechnology and Genetic Engineering, Faisalabad, Pakistan.
Umair Hassan KhanSchool of Agriculture and Environment, The University of Western Australia, Crawley, Perth, Australia.
Azra YasminMicrobiology and Biotechnology Laboratory, Department of Environmental Sciences, Fatima Jinnah Women University, Rawalpindi, 46000, Pakistan.
Fatima Jinnah Women University · PKNational Institute for Biotechnology and Genetic Engineering · PKThe University of Western Australia · AUUniversity of Okara · PK

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This study explores the chemotactic potential of Bacillus subtilis MB378 against industrial dyes. Initial screening with swim plate assay showed significant movement of Bacillus subtilis MB378 towards test compounds. According to quantitative capillary assay, B. subtilis MB378 exhibited high chemotaxis potential towards Acid Orange 52 (CI: 9.52), followed by Direct Red 28 (CI: 8.39) and Basic Green 4 (CI: 5.21) in glucose-supplemented medium. Sequencing and gene annotation results evidently showed presence of chemotaxis genes and flagellar motor proteins in Bacillus subtilis draft genome. Methyl-accepting proteins (involved in chemotaxis regulation) belonged to pfam00672, pfam00072, and pfam00015 protein families. Annotated chemotaxis machinery of MB378 comprised 8 Che genes, 5 chemoreceptor genes, associated flagellar proteins, and rotary motors. Chemotaxis genes of B. subtilis MB378 were compared with genes of closely related Bacillus strains (168, WK1, and HTA426), depicting highly conserved regions showing evolutionary relation between them. Considering results of present study, it can be speculated that test compounds triggered chemotactic genes, which made these compounds bioavailable to the bacterium. Hence, the bacterium recognized and approached these compounds and facilitated biodegradation and detoxification of these compounds.

Indexed as

Bacillus subtilisChemotaxisWater DecolorizationBacterial ProteinsColoring AgentsMembrane ProteinsMethyl-Accepting Chemotaxis ProteinsMultigene FamilyBacterial ProteinsColoring AgentsMembrane ProteinsMethyl-Accepting Chemotaxis ProteinsBacillus subtilisChemotaxis machineryMethyl-accepting proteinsMultiple sequence alignmentWhole genome annotation

Identifiers

PMID34389949
OpenAlexW3191471901

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.