ArticleeLife2021
Scaled, high fidelity electrophysiological, morphological, and transcriptomic cell characterization.
Article in eLife, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
45 citing papers in PubMed.
- Article
- Adaptive Network Stabilization Despite Profound Structural Reorganization in Human Organotypic Cultures.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- A framework for comparative analysis of human and mouse cortical neuron dendrites in corresponding brain regions.Nature neuroscience · 2026Article
- Aspiration Patch Proteomics Enables CE-ESI-MS Proteotyping of Identified Single Neurons in Intact Brain Tissue.Analytical chemistry · 2026Article
- Multimodal characterization of variation in neuronal types in the mouse basal ganglia.bioRxiv : the preprint server for biology · 2026Article
- Single-cell RNA sequencing in stroke and traumatic brain injury: Current achievements, challenges and future perspectives on transcriptomic profiling.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2026Review
- Morphoelectric Diversity and Specialization of Neuronal Cell Types in the Primate Striatum.bioRxiv : the preprint server for biology · 2026Article
- HCN channels reveal conserved and divergent physiology in supragranular pyramidal neurons in primate species.Communications biology · 2026Article
- Human Neocortical Glutamatergic Neurons Revealed Through Multimodal Profiling.bioRxiv : the preprint server for biology · 2026Article
- Convergent Control of NREM Sleep and Anesthesia by Prefrontal Layer 5 Extratelencephalic Neurons.Research square · 2025Article
- A Spinal Circuit for Modular Gating of Organ Somatosensation.bioRxiv : the preprint server for biology · 2025Article
- In Search of Transcriptomic Correlates of Neuronal Firing-Rate Adaptation across Subtypes, Regions and Species: A Patch-seq Analysis.bioRxiv : the preprint server for biology · 2025Article
- Article
- Article
- Principles of visual cortex excitatory microcircuit organization.Innovation (Cambridge (Mass.)) · 2025Article
- Article
- Distinctive physiology of molecularly identified medium spiny neurons in the macaque putamen.Cell reports · 2024Article
- VTA dopamine neurons are hyperexcitable in 3xTg-AD mice due to casein kinase 2-dependent SK channel dysfunction.Nature communications · 2024Article
- Areal specializations in the morpho-electric and transcriptomic properties of primate layer 5 extratelencephalic projection neurons.Cell reports · 2024Article
- Protocol for mapping cell lineage and cell-type identity of clonally-related cells in situ using MADM-CloneSeq.STAR protocols · 2024Article
Corrections and comments
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Authors and funding
28 authors.
Funding
Abstract
The Patch-seq approach is a powerful variation of the patch-clamp technique that allows for the combined electrophysiological, morphological, and transcriptomic characterization of individual neurons. To generate Patch-seq datasets at scale, we identified and refined key factors that contribute to the efficient collection of high-quality data. We developed patch-clamp electrophysiology software with analysis functions specifically designed to automate acquisition with online quality control. We recognized the importance of extracting the nucleus for transcriptomic success and maximizing membrane integrity during nucleus extraction for morphology success. The protocol is generalizable to different species and brain regions, as demonstrated by capturing multimodal data from human and macaque brain slices. The protocol, analysis and acquisition software are compiled at https://githubcom/AllenInstitute/patchseqtools. This resource can be used by individual labs to generate data across diverse mammalian species and that is compatible with large publicly available Patch-seq datasets.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.