Evidence map›Paper›PMID 34386960›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2021

Preparation and Characterization of Quantum Dot-Peptide Conjugates Based on Polyhistidine Tags.

Katherine D Krause, Hsin-Yun Tsai, Kelly Rees, Hyungki Kim, W Russ Algar

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Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Katherine D KrauseDepartment of Chemistry, University of British Columbia, Vancouver, BC, Canada.
Hsin-Yun TsaiDepartment of Chemistry, University of British Columbia, Vancouver, BC, Canada.
Kelly ReesDepartment of Chemistry, University of British Columbia, Vancouver, BC, Canada.
Hyungki KimDepartment of Chemistry, University of British Columbia, Vancouver, BC, Canada.
W Russ AlgarDepartment of Chemistry, University of British Columbia, Vancouver, BC, Canada. algar@chem.ubc.ca.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Quantum dots (QDs) offer bright and robust photoluminescence among several other advantages in comparison to fluorescent dyes. In order to leverage the advantageous properties of QDs for applications in bioanalysis and imaging, simple and reliable methods for bioconjugation are required. One such method for conjugating peptides to QDs is the use of polyhistidine tags, which spontaneously bind to the surface of QDs. We describe protocols for assembling polyhistidine-tagged peptides to QDs and for characterizing the resultant QD-peptide conjugates. The latter include both electrophoretic and FRET-based protocols for confirming successful peptide assembly, estimating the maximum peptide loading capacity, and measuring the assembly kinetics. Sensors for protease activity and intracellular delivery are briefly noted as prospective applications of QD-peptide conjugates.

Indexed as

Quantum DotsFluorescence Resonance Energy TransferHistidineHistidinepolyhistidineAgarose gel electrophoresisFörster resonance energy transfer (FRET)PeptidesPolyacrylamide gel electrophoresisPolyhistidineQuantum dots

Identifiers

PMID34386960

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.