Evidence map›Paper›PMID 34385528›Full record

ArticleScientific reports2021

shRNA transgenic swine display resistance to infection with the foot-and-mouth disease virus.

Wenping Hu, Haixue Zheng, Qiuyan Li, Yuhang Wang, Xiangtao Liu, Xiaoxiang Hu, Wenjie Liu, Shen Liu, Zhisheng Chen, Wenhai Feng and 2 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.0field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 5 citations in OpenAlex.

  1. Review
  2. Pig Genome Editing for Agriculture: Achievements and Challenges.International journal of molecular sciences · 2025
    Review
  3. Article
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 4 institutions in 1 country.

Wenping Hu *State Key Laboratory of AgroBiotechnology, China Agricultural University, Beijing, China.
Haixue Zheng *State Key Laboratory of Veterinary Etiological Biology, National Foot and Mouth Diseases Reference Laboratory, Lanzhou Veterinarian Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu, China.
Qiuyan LiState Key Laboratory of AgroBiotechnology, China Agricultural University, Beijing, China.
Yuhang WangState Key Laboratory of AgroBiotechnology, China Agricultural University, Beijing, China.
Xiangtao LiuState Key Laboratory of Veterinary Etiological Biology, National Foot and Mouth Diseases Reference Laboratory, Lanzhou Veterinarian Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu, China.
Xiaoxiang HuState Key Laboratory of AgroBiotechnology, China Agricultural University, Beijing, China.
Wenjie LiuState Key Laboratory of AgroBiotechnology, China Agricultural University, Beijing, China.
Shen LiuState Key Laboratory of AgroBiotechnology, China Agricultural University, Beijing, China.
Zhisheng ChenState Key Laboratory of AgroBiotechnology, China Agricultural University, Beijing, China.
Wenhai FengState Key Laboratory of AgroBiotechnology, China Agricultural University, Beijing, China.
Xuepeng CaiState Key Laboratory of Veterinary Etiological Biology, National Foot and Mouth Diseases Reference Laboratory, Lanzhou Veterinarian Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu, China. caixp@vip.163.com.
Ning LiState Key Laboratory of AgroBiotechnology, China Agricultural University, Beijing, China. ninglcau@sina.com.
China Agricultural University · CNChinese Academy of Agricultural Sciences · CNInstitute of Animal Sciences · CNLanzhou Veterinary Research Institute · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Foot-and-mouth disease virus (FMDV) is one of the most important animal pathogens in the world. FMDV naturally infects swine, cattle, and other cloven-hoofed animals. FMD is not adequately controlled by vaccination. An alternative strategy is to develop swine that are genetically resistant to infection. Here, we generated FMDV-specific shRNA transgenic cells targeting either nonstructural protein 2B or polymerase 3D of FMDV. The shRNA-positive transgenic cells displayed significantly lower viral production than that of the control cells after infection with FMDV (P < 0.05). Twenty-three transgenic cloned swine (TGCS) and nine non-transgenic cloned swine (Non-TGCS) were produced by somatic cell nuclear transfer (SCNT). In the FMDV challenge study, one TGCS was completely protected, no clinical signs, no viremia and no viral RNA in the tissues, no non-structural antibody response, another one TGCS swine recovered after showing clinical signs for two days, whereas all of the normal control swine (NS) and Non-TGCS developed typical clinical signs, viremia and viral RNA was determined in the tissues, the non-structural antibody was determined, and one Non-TGCS swine died. The viral RNA load in the blood and tissues of the TGCS was reduced in both challenge doses. These results indicated that the TGCS displayed resistance to the FMDV infection. Immune cells, including CD3

Indexed as

AnimalsAnimals, Genetically ModifiedDisease ResistanceFoot-and-Mouth DiseaseFoot-and-Mouth Disease VirusRNA InterferenceRNA, Small InterferingRNA, ViralSwineSwine DiseasesViremiaRNA, Small InterferingRNA, Viral

Identifiers

PMID34385528
PMCPMC8361160
OpenAlexW3187327102

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.