Evidence map›Paper›PMID 34384841›Full record

ArticleThe Journal of allergy and clinical immunology2022

Poor T-cell receptor β repertoire diversity early posttransplant for severe combined immunodeficiency predicts failure of immune reconstitution.

Ottavia M Delmonte, Riccardo Castagnoli, Jason Yu, Christopher C Dvorak, Morton J Cowan, Blachy J Dávila Saldaña, Suk See De Ravin, Ewelina Mamcarz, Catherine K Chang, Stephen R Daley and 3 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of allergy and clinical immunology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.9field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 12 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 5 institutions in 3 countries.

Ottavia M DelmonteLaboratory of Clinical Immunology and Microbiology, NIAID, NIH, Bethesda, Md.
Riccardo CastagnoliLaboratory of Clinical Immunology and Microbiology, NIAID, NIH, Bethesda, Md.
Jason YuDivision of Allergy, Immunology and Blood and Marrow Transplantation, Department of Pediatrics, University of California San Francisco School of Medicine, San Francisco, Calif; Smith Cardiovascular Research Institute, San Francisco, Calif; UCSF Benioff Children's Hospital, San Francisco, Calif.
Christopher C DvorakDivision of Allergy, Immunology and Blood and Marrow Transplantation, Department of Pediatrics, University of California San Francisco School of Medicine, San Francisco, Calif; UCSF Benioff Children's Hospital, San Francisco, Calif.
Morton J CowanDivision of Allergy, Immunology and Blood and Marrow Transplantation, Department of Pediatrics, University of California San Francisco School of Medicine, San Francisco, Calif; Smith Cardiovascular Research Institute, San Francisco, Calif; UCSF Benioff Children's Hospital, San Francisco, Calif.
Blachy J Dávila SaldañaDivision of Blood and Marrow Transplantation, Children's National Medical Center, Washington, DC.
Suk See De RavinLaboratory of Clinical Immunology and Microbiology, NIAID, NIH, Bethesda, Md.
Ewelina MamcarzDepartment of Bone Marrow Transplantation and Cellular Therapy, St Jude Children's Research Hospital, Memphis, Tenn.
Catherine K ChangDivision of Allergy, Immunology and Blood and Marrow Transplantation, Department of Pediatrics, University of California San Francisco School of Medicine, San Francisco, Calif; UCSF Benioff Children's Hospital, San Francisco, Calif.
Stephen R DaleyCentre for Immunology and Infection Control, School of Biomedical Sciences, Queensland University of Technology, Brisbane, Australia.
Linda M GriffithDivision of Allergy Immunology and Transplantation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Md.
Luigi D NotarangeloLaboratory of Clinical Immunology and Microbiology, NIAID, NIH, Bethesda, Md. Electronic address: luigi.notarangelo2@nih.gov.
Jennifer M PuckDivision of Allergy, Immunology and Blood and Marrow Transplantation, Department of Pediatrics, University of California San Francisco School of Medicine, San Francisco, Calif; Smith Cardiovascular Research Institute, San Francisco, Calif; UCSF Benioff Children's Hospital, San Francisco, Calif. Electronic address: Jennifer.Puck@ucsf.edu.
National Institutes of Health · USUniversity of California, San Francisco · USChildren's National · USQueensland University of Technology · AUSt. Jude Children's Research Hospital · US

Funding

Training of Clinical Investigators in Primary Immune DeficienciesU54AI082973 · NIAID · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI DVORAK, CHRISTOPHER CRAIG, HADDAD, ELIE · 2009 to 2023
$23.3M
Characterization of the cellular and molecular bases of inborn errors of immunityZIAAI001222 · NIAID · NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES · PI NOTARANGELO, LUIGI · 2017 to 2025
$15.2M
Inherited T cell defects: Diagnosis, Mechanisms and TreatmentsP01AI138962 · NIAID · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI WIEST, DAVID L. · 2020 to 2024
$11.3M
Development and Clinical Application of Gene and Cell therapies for Patients with Immune DeficienciesZIAAI000645 · NIAID · NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES · PI DE RAVIN, SUK SEE · 2009 to 2025
$10.1M
Primary Immune Deficiency Treatment Consortium Annual Scientific MeetingR13AI094943 · NIAID · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI PUCK, JENNIFER M. · 2011 to 2025
$239k
Intramural NIH HHS ZIA AI001222NIAID NIH HHS P01 AI138962NIAID NIH HHS R13 AI094943NIAID NIH HHS U54 AI082973
6 · The paper itself

Abstract

backgroundDevelopment of a diverse T-cell receptor β (TRB) repertoire is associated with immune recovery following hematopoietic cell transplantation (HCT) for severe combined immunodeficiency (SCID). High-throughput sequencing of the TRB repertoire allows evaluation of clonotype dynamics during immune reconstitution.

objectivesWe investigated whether longitudinal analysis of the TRB repertoire would accurately describe T-cell receptor diversity and illustrate the quality of T-cell reconstitution following HCT or gene therapy for SCID.

methodsWe used high-throughput sequencing to study composition and diversity of the TRB repertoire in 27 infants with SCID at 3, 6, and 12 months and yearly posttreatment(s). Total RNA from peripheral blood was used as template to amplify TRB rearrangements.

resultsTRB sequence analysis showed poor diversity at 3 months, followed by significant improvement by 6 months after cellular therapies. Kinetics of development of TRB diversity were similar in patients with a range of underlying gene defects. However, in patients with RAG and DCLRE1C defects, HCT with no conditioning or immune suppression only resulted in lower diversity than did HCT with conditioning. HCT from a matched donor correlated with higher diversity than did HCT from a mismatched donor. Naive CD4

conclusionsTRB repertoire after hematopoietic cell therapies for SCID provides a quantitative and qualitative measure of diversity of T-cell reconstitution and permits early identification of patients who may require a second intervention.

Indexed as

Hematopoietic Stem Cell TransplantationImmune ReconstitutionSevere Combined ImmunodeficiencyComplementarity Determining RegionsHumansInfantReceptors, Antigen, T-CellReceptors, Antigen, T-Cell, alpha-betaComplementarity Determining RegionsReceptors, Antigen, T-CellReceptors, Antigen, T-Cell, alpha-betaComplementarity determining region 3 (CDR3)hematopoietic cell transplantationhigh-throughput sequencingimmune reconstitutionsevere combined immunodeficiency (SCID)T-cell receptor (TCR)T-cell receptor β (TRB) diversityT-cell receptor β (TRB) repertoire

Identifiers

PMID34384841
PMCPMC9132846
OpenAlexW3187152632

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.