ArticleJournal of virological methods2021
Use of guanidine thiocyanate-based nucleic acid extraction buffers to inactivate poliovirus in potentially infectious materials.
Article in Journal of virological methods, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 17 citations in OpenAlex.
- Genomics in containment: BSL-4-compatible workflows enable high-resolution genomic and transcriptomic analyses of Risk Group 4 viruses.iScience · 2026Article
- Assessment of human health risks to tick-borne infections in urban green spaces (UGS) - a study protocol.BMC infectious diseases · 2025Article
- Implementation of Poliovirus Containment in Poliovirus Designated Facilities-United States, 2017-2024.Pathogens (Basel, Switzerland) · 2025Article
- Evaluating viral inactivation in the liquid waste stream from a viral total nucleic acid extraction kit for safe disposal.Biosafety and health · 2025Article
- Effect of temperature and time on RNA detection by RT-qPCR in rodent tissue and blood samples stored in MagMAX™ Lysis/Binding Solution Concentrate: Considerations for RNA detection in specimens stored under suboptimal conditions.Journal of virological methods · 2025Article
- A rapid and high-yield method for nucleic acid extraction.Scientific reports · 2025Article
- Inactivation of highly transmissible livestock and avian viruses including influenza A and Newcastle disease virus for molecular diagnostics.Frontiers in veterinary science · 2024Article
- Evaluation of Direct Detection Protocols for Poliovirus from Stool Samples of Acute Flaccid Paralysis Patients.Viruses · 2023Article
- How to better select SARS-CoV-2 preservation solution of virus nucleic acid testing.Journal of clinical laboratory analysis · 2023Article
- Culture-Independent Detection of Poliovirus in Stool Samples by Direct RNA Extraction.Microbiology spectrum · 2021Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
The efforts of the Global Poliovirus Eradication Initiative (GPEI) have brought about the near elimination of poliovirus worldwide. The World Health Organization has issued guidelines for the safe handling and containment of infectious materials (IM) and potentially infectious materials (PIM) following poliovirus eradication. Inactivation of poliovirus in IM and PIM is needed to prevent inadvertent re-introduction of polioviruses post-eradication. In this study, we investigated the use of guanidine thiocyanate-based nucleic acid extraction buffers from commercially available nucleic acid extraction kits to inactivate poliovirus in cell culture isolates and stool suspensions, two common types of poliovirus IM and PIM, respectively. Incubation with selected nucleic acid extraction buffers or extraction buffers supplemented with ethanol reduced the infectivity of high-titer wild poliovirus type 1 (WPV1), wild poliovirus type 3 (WPV3), Sabin 1 (SL1), and Sabin 3 (SL3) cell culture isolates below the limit of detection in CCID
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.