ArticleParasites & vectors2021
Blastocystis in the faeces of children from six distant countries: prevalence, quantity, subtypes and the relation to the gut bacteriome.
Article in Parasites & vectors, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Effects of Lactiplantibacillus plantarum and Lacticaseibacillus paracasei supplementation on the single-cell fecal parasitome in children with celiac disease autoimmunity: a randomized, double-blind placebo-controlled clinical trial.Parasites & vectors · 2023Trial
- Molecular characterization of Blastocystis sp., Cryptosporidium spp., and Giardia spp. in Eld's deer (Rucervus eldii) and their forest rangers in Hainan, China.Parasites & vectors · 2026Article
- Prevalence and Subtypes of Blastocystis in Symptomatic Children in Egypt and Anti-Blastocystis Efficacy of Artemisia alba-Loaded Silver Nanoparticles In Vitro and in Swiss Albino Mice.Acta parasitologica · 2026Article
- Human Gut Microbiota Profiles Related to Mediterranean and West African Diets and Association withNutrients · 2025Article
- Epidemiological survey on prevalence and subtypes distribution of Blastocystis sp. in Southern Guizhou, China.Biomolecules & biomedicine · 2025Article
- Impact of the SARS-CoV-2 Pandemic on the Prevalence and Incidence of Enteric Protozoa in a Spanish Tertiary-Care Hospital and a Referral Center for Tropical Diseases, 2019-2023.Medical sciences (Basel, Switzerland) · 2025Article
- Updated report of Blastocystis spp. in a teaching hospital of Brazil: A 6-year retrospective experience.Clinics (Sao Paulo, Brazil) · 2025Article
- Investigation of gut microbiota composition in humans carrying blastocystis subtypes 1 and 2 and Entamoeba hartmanni.Gut pathogens · 2024Article
- Blastocystis hominis; Friend or Foe.Integrative medicine (Encinitas, Calif.) · 2024Review
- Molecular investigation of Blastocystis in children and calves in Bangladesh.BMC microbiology · 2024Article
- Molecular Prevalence and Subtypes Distribution ofTropical medicine and infectious disease · 2024Review
- Gastrointestinal Pathogens in Multi-Infected Individuals: A Cluster Analysis of Interaction.Microorganisms · 2023Article
- Epidemiology ofPathogens (Basel, Switzerland) · 2023Review
- Exploring Blastocystis genetic diversity in rural schoolchildren from Colombia using next-generation amplicon sequencing reveals significant associations between contact with animals and infection risk.Parasitology research · 2023Article
- A longitudinal study of Blastocystis in dairy calves from birth through 24 months demonstrates dynamic shifts in infection rates and subtype prevalence and diversity by age.Parasites & vectors · 2023Article
- Maternal and food microbial sources shape the infant microbiome of a rural Ethiopian population.Current biology : CB · 2023Article
- Subtypes and phylogenetic analysis of Blastocystis sp. isolates from West Ismailia, Egypt.Scientific reports · 2022Article
- Rare Occurrence ofJournal of clinical medicine · 2022Article
- Detection, Molecular Identification and Transmission of the Intestinal ProtozoaMicroorganisms · 2022Article
- The regulatory function ofFrontiers in cellular and infection microbiology · 2022Review
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13 authors.
Funding
Abstract
backgroundBlastocystis is a human gut symbiont of yet undefined clinical significance. In a set of faecal samples collected from asymptomatic children of six distant populations, we first assessed the community profiles of protist 18S rDNA and then characterized Blastocystis subtypes and tested Blastocystis association with the faecal bacteriome community.
methodsStool samples were collected from 244 children and young persons (mean age 11.3 years, interquartile range 8.1-13.7) of six countries (Azerbaijan 51 subjects, Czechia 52, Jordan 40, Nigeria 27, Sudan 59 and Tanzania 15). The subjects showed no symptoms of infection. Amplicon profiling of the 18S rDNA was used for verification that Blastocystis was the most frequent protist, whereas specific real-time PCR showed its prevalence and quantity, and massive parallel amplicon sequencing defined the Blastocystis subtypes. The relation between Blastocystis and the stool bacteriome community was characterized using 16S rDNA profiling.
resultsBlastocystis was detected by specific PCR in 36% (88/244) stool samples and was the most often observed faecal protist. Children from Czechia and Jordan had significantly lower prevalence than children from the remaining countries. The most frequent subtype was ST3 (49%, 40/81 sequenced samples), followed by ST1 (36%) and ST2 (25%). Co-infection with two different subtypes was noted in 12% samples. The faecal bacteriome had higher richness in Blastocystis-positive samples, and Blastocystis was associated with significantly different community composition regardless of the country (p < 0.001 in constrained redundancy analysis). Several taxa differed with Blastocystis positivity or quantity: two genera of Ruminococcaceae were more abundant, while Bifidobacterium, Veillonella, Lactobacillus and several other genera were undrerrepresented.
conclusionsAsymptomatic children frequently carry Blastocystis, and co-infection with multiple distinct subtypes is not exceptional. Prevalence and quantity of the organism clearly differ among populations. Blastocystis is linked to both faecal bacteriome diversity and its composition.
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