Evidence map›Paper›PMID 34384463›Full record

ArticleBiotechnology for biofuels2021

CAZymes from the thermophilic fungus Thermoascus aurantiacus are induced by C5 and C6 sugars.

Raphael Gabriel, Rebecca Mueller, Lena Floerl, Cynthia Hopson, Simon Harth, Timo Schuerg, Andre Fleissner, Steven W Singer

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biotechnology for biofuels, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.9field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 15 citations in OpenAlex.

  1. Article
  2. Article
  3. A Combined Study on Optimization,Biochemistry research international · 2022
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 4 institutions in 5 countries.

Raphael GabrielBiological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 9720, USA.
Rebecca MuellerBiological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 9720, USA.
Lena FloerlBiological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 9720, USA.
Cynthia HopsonBiological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 9720, USA.
Simon HarthBiological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 9720, USA.
Timo SchuergBiological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 9720, USA.
Andre FleissnerInstitut Für Genetik, Technische Universität Braunschweig, Spielmannstr. 7, 38106, Braunschweig, Germany.
Steven W SingerBiological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 9720, USA. swsinger@lbl.gov.ORCID http://orcid.org/0000-0002-4229-8314
Lawrence Berkeley National Laboratory · USGoethe University Frankfurt · DETechnische Universität Braunschweig · DEUniversidad Complutense de Madrid · ES

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundFilamentous fungi are excellent lignocellulose degraders, which they achieve through producing carbohydrate active enzymes (CAZymes). CAZyme production is highly orchestrated and gene expression analysis has greatly expanded understanding of this important biotechnological process. The thermophilic fungus Thermoascus aurantiacus secretes highly active thermostable enzymes that enable saccharifications at higher temperatures; however, the genome-wide measurements of gene expression in response to CAZyme induction are not understood.

resultsA fed-batch system with plant biomass-derived sugars D-xylose, L-arabinose and cellobiose established that these sugars induce CAZyme expression in T. aurantiacus. The C5 sugars induced both cellulases and hemicellulases, while cellobiose specifically induced cellulases. A minimal medium formulation was developed to enable gene expression studies of T. aurantiacus with these inducers. It was found that d-xylose and L-arabinose strongly induced a wide variety of CAZymes, auxiliary activity (AA) enzymes and carbohydrate esterases (CEs), while cellobiose facilitated lower expression of mostly cellulase genes. Furthermore, putative orthologues of different unfolded protein response genes were up-regulated during the C5 sugar feeding together with genes in the C5 sugar assimilation pathways.

conclusionThis work has identified two additional CAZyme inducers for T. aurantiacus, L-arabinose and cellobiose, along with D-xylose. A combination of biochemical assays and RNA-seq measurements established that C5 sugars induce a suite of cellulases and hemicellulases, providing paths to produce broad spectrum thermotolerant enzymatic mixtures.

Indexed as

CAZyCellulase gene expressionFilamentous fungiThermoascus aurantiacusTranscriptomics

Identifiers

PMID34384463
PMCPMC8359064
OpenAlexW3190448967

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.