Evidence map›Paper›PMID 34383224›Full record

ArticleWiener medizinische Wochenschrift (1946)2021

Food additive E407a stimulates eryptosis in a dose-dependent manner.

Anton Tkachenko, Yurii Kot, Volodymyr Prokopyuk, Anatolii Onishchenko, Alla Bondareva, Valeriy Kapustnik, Tetyana Chumachenko, Yevgen Perskiy, Dmytro Butov, Oksana Nakonechna

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Article in Wiener medizinische Wochenschrift (1946), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

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2citing papers in PubMed
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1 · What the graph read from it

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3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Cytotoxicity of Hybrid Noble Metal-Polymer Composites.BioMed research international · 2022
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4 · The record

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5 · Who and what money

Authors and funding

10 authors.

Anton TkachenkoResearch Institute of Experimental and Clinical Medicine, Kharkiv National Medical University, Nauky ave 4, 61022, Kharkiv, Ukraine. antontkachenko555@gmail.com.ORCID http://orcid.org/0000-0002-1029-1636
Yurii KotDepartment of Biochemistry, V.N. Karazin Kharkiv National University, 61022, Kharkiv, Ukraine.
Volodymyr ProkopyukResearch Institute of Experimental and Clinical Medicine, Kharkiv National Medical University, Nauky ave 4, 61022, Kharkiv, Ukraine.
Anatolii OnishchenkoResearch Institute of Experimental and Clinical Medicine, Kharkiv National Medical University, Nauky ave 4, 61022, Kharkiv, Ukraine.
Alla BondarevaDepartment of Biochemistry, Kharkiv National Medical University, 61022, Kharkiv, Ukraine.
Valeriy KapustnikDepartment of Internal and Occupational Diseases, Kharkiv National Medical University, 61022, Kharkiv, Ukraine.
Tetyana ChumachenkoDepartment of Epidemiology, Kharkiv National Medical University, 61022, Kharkiv, Ukraine.
Yevgen PerskiyDepartment of Biochemistry, V.N. Karazin Kharkiv National University, 61022, Kharkiv, Ukraine.
Dmytro ButovDepartment of Phthisiology and Pulmonology, Kharkiv National Medical University, 61022, Kharkiv, Ukraine.
Oksana NakonechnaDepartment of Biochemistry, Kharkiv National Medical University, 61022, Kharkiv, Ukraine.

Funding

Ministry of Health of Ukraine 0118U000943
6 · The paper itself

Abstract

backgroundConcerns about the biosafety of the common food additive E407a have been raised. It has been demonstrated to induce intestinal inflammation, accompanied by activation of apoptosis, upon oral exposure. Thus, it is of interest to investigate how E407a affects eryptosis, a suicidal cell death mode of red blood cells.

objectiveTo evaluate the effects of semi-refined carrageenan (E407a) on eryptosis.

methodsFlow cytometry was employed to assess eryptosis in blood exposed to various concentrations of E407a (0 g/L, 1 g/L, 5 g/L, and 10 g/L) during incubation for 24 h by analyzing phosphatidylserine externalization in erythrocytes using annexin V staining and via evaluating reactive oxygen species (ROS) generation using 2',7'-dichlorodihydrofluorescein diacetate (H

resultsOral intake of E407a for 2 weeks by rats was not associated with membrane scrambling in erythrocytes. However, ROS overproduction was observed. Meanwhile, incubation of blood with various concentrations of semi-refined carrageenan resulted in a dose-dependent promotion of eryptosis, evidenced by the enhanced percentage of annexin V-positive erythrocytes and higher mean fluorescence intensity (MFI) values of annexin V-FITC in all erythrocytes. The highest concentration of E407a promotes a statistically significant increase in ROS generation in erythrocytes, suggesting the role of ROS-mediated induction of eryptosis in this case.

conclusionIncubation of blood with the food additive E407a leads to the activation of eryptosis in a dose-dependent manner. ROS-mediated mechanisms are partially responsible for E407a-induced eryptosis.

Indexed as

Annexin VConfocal microscopyFlow cytometryProcessed Eucheuma seaweedReactive oxygen species

Identifiers

PMID34383224

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