Evidence map›Paper›PMID 34383168›Full record

ArticleJournal of fluorescence2021

A Nitronaphthalimide Probe for Fluorescence Imaging of Hypoxia in Cancer Cells.

Rashmi Kumari, Vasumathy R, Dhanya Sunil, Raghumani Singh Ningthoujam, Badri Narain Pandey, Suresh D Kulkarni, Thivaharan Varadavenkatesan, Ganesh Venkatachalam, Anil Kumar N V

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of fluorescence, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.7field-weighted citation impact, top 35% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 12 citations in OpenAlex.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Rashmi KumariDepartment of Chemistry, Manipal Institute of Technology, Manipal Academy of Higher Education, Manipal, 576104, Karnataka, India.
Vasumathy RRadiation Biology & Health Sciences Division, Bhabha Atomic Research Centre, Mumbai, 400085, Maharashtra, India.
Dhanya SunilDepartment of Chemistry, Manipal Institute of Technology, Manipal Academy of Higher Education, Manipal, 576104, Karnataka, India. dhanya.s@manipal.edu.ORCID http://orcid.org/0000-0002-0727-5125
Raghumani Singh NingthoujamChemistry Division, Bhabha Atomic Research Centre, Mumbai, 400085, Maharashtra, India.
Badri Narain PandeyRadiation Biology & Health Sciences Division, Bhabha Atomic Research Centre, Mumbai, 400085, Maharashtra, India.
Suresh D KulkarniDepartment of Atomic and Molecular Physics, Manipal Academy of Higher Education, Manipal, 576104, Karnataka, India.
Thivaharan VaradavenkatesanDepartment of Biotechnology, Manipal Institute of Technology, Manipal Academy of Higher Education, Manipal, 576104, Karnataka, India.
Ganesh VenkatachalamElectrodics and Electrocatalysis (EEC) Division, CSIR - Central Electrochemical Research Institute (CSIR-CECRI), Karaikudi, 630003, Tamil Nadu, India.
Anil Kumar N VDepartment of Chemistry, Manipal Institute of Technology, Manipal Academy of Higher Education, Manipal, 576104, Karnataka, India.
Manipal Academy of Higher Education · INBhabha Atomic Research Centre · INCentral Electrochemical Research Institute · IN

Funding

department of atomic energy, government of india 37(2)/14/01/2018-BRNS/37001
6 · The paper itself

Abstract

The bioreductive enzymes typically upregulated in hypoxic tumor cells can be targeted for developing diagnostic and drug delivery applications. In this study, a new fluorescent probe 4-(6-nitro-1,3-dioxo-1H-benzo[de]isoquinolin-2(3H)-yl)benzaldehyde (NIB) based on a nitronaphthalimide skeleton that could respond to nitroreductase (NTR) overexpressed in hypoxic tumors is designed and its application in imaging tumor hypoxia is demonstrated. The docking studies revealed favourable interactions of NIB with the binding pocket of NTR-Escherichia coli. NIB, which is synthesized through a simple and single step imidation of 4-nitro-1,8-naphthalic anhydride displayed excellent reducible capacity under hypoxic conditions as evidenced from cyclic voltammetry investigations. The fluorescence measurements confirmed the formation of identical products (NIB-red) during chemical as well as NTR-aided enzymatic reduction in the presence of NADH. The potential fluorescence imaging of hypoxia based on NTR-mediated reduction of NIB is confirmed using in-vitro cell culture experiments using human breast cancer (MCF-7) cells, which displayed a significant change in the fluorescence colour and intensity at low NIB concentration within a short incubation period in hypoxic conditions.

Indexed as

Optical ImagingCell HypoxiaFluorescent DyesHumansMCF-7 CellsMolecular Docking SimulationNitroreductasesTumor Cells, CulturedFluorescent DyesNitroreductasesDockingFluorescenceHypoxiaMolecular probeNitronaphthalimideNitroreductase

Identifiers

PMID34383168
PMCPMC8545720
OpenAlexW3193124883

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.