Evidence map›Paper›PMID 34382118›Full record

ArticleOdontology2022

Characterization of a clonal human periodontal ligament stem cell line exposed to methacrylate resin-, bioactive glass-, or silicon-based root canal sealers.

Atsushi Tomokiyo, Daigaku Hasegawa, Taiga Ono, Ryoko Nagano, Keita Ipposhi, Kozue Yamashita, M Anas Alhasan, Hidefumi Maeda

Abstract read
PubMed Publisher
In one paragraph

Article in Odontology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Atsushi TomokiyoDepartment of Endodontology, Kyushu University Hospital, 3-1-1 Maidashi, Higashi-ku, Fukuoka-shi, Fukuoka, 812 8582, Japan. tomokiyo@dent.kyushu-u.ac.jp.ORCID http://orcid.org/0000-0002-1638-4008
Daigaku HasegawaDepartment of Endodontology, Kyushu University Hospital, 3-1-1 Maidashi, Higashi-ku, Fukuoka-shi, Fukuoka, 812 8582, Japan.
Taiga OnoFaculty of Dental Science, Division of Oral Rehabilitation, Department of Endodontology and Operative Dentistry, Kyushu University, Fukuoka, Japan.
Ryoko NaganoFaculty of Dental Science, Division of Oral Rehabilitation, Department of Endodontology and Operative Dentistry, Kyushu University, Fukuoka, Japan.
Keita IpposhiFaculty of Dental Science, Division of Oral Rehabilitation, Department of Endodontology and Operative Dentistry, Kyushu University, Fukuoka, Japan.
Kozue YamashitaFaculty of Dental Science, Division of Oral Rehabilitation, Department of Endodontology and Operative Dentistry, Kyushu University, Fukuoka, Japan.
M Anas AlhasanFaculty of Dental Science, Division of Oral Rehabilitation, Department of Endodontology and Operative Dentistry, Kyushu University, Fukuoka, Japan.
Hidefumi MaedaDepartment of Endodontology, Kyushu University Hospital, 3-1-1 Maidashi, Higashi-ku, Fukuoka-shi, Fukuoka, 812 8582, Japan.

Funding

grant-in-aid for scientific research from the japan society for the promotion of science JP21K09876grant-in-aid for scientific research from the japan society for the promotion of science JP21K16994Grant-in-Aid for Scientific Research fromthe Japan Society for the Promotion of Science JP 17H04385
6 · The paper itself

Abstract

The aim of this study was to characterize a clonal human periodontal ligament (PDL) stem cell line (line 2-23 cells) cultured with root canal sealers based on methacrylate resin (SuperBond sealer; SB), bioactive glass (Nishika Canal Sealer BG; BG), or silicon (GuttaFlow 2; GF). The sealers were set in rubber molds to form sealer discs. Line 2-23 cells were cultured with or without the discs for 3 days. The cell viability was evaluated by direct cell counting and MTT assay. Inflammation-, PDL-, collagen-, and cell cycle-related gene expression was investigated by real-time RT-PCR. Collagen production was analyzed by Picro Sirius Red staining. Calcium ion concentration in the culture was measured by a QuantiChrom calcium assay kit. Line 2-23 cells survived when cultured with GF discs, but decreased cell viability was observed with SB and BG discs. The expression of inflammation-related genes was higher in cells cultured with SB discs, and expression of PDL-related genes was lower in cells exposed to SB and BG discs. These discs also down-regulated collagen production in line 2-23 cells. BG discs increased calcium ion concentration in the culture medium. Cells exposed to GF discs exhibited the same inflammation-, PDL-, collagen-, and cell cycle-related gene expression and collagen production as untreated cells. These results suggested that the characteristics of line 2-23 cells cultured with GF discs was highly resemble to untreated cells throughout the 3 days of the culture model.

Indexed as

Root Canal Filling MaterialsSiliconCell LineDental Pulp CavityEpoxy ResinsHumansMaterials TestingMethacrylatesPeriodontal LigamentEpoxy ResinsMethacrylatesRoot Canal Filling MaterialsSiliconBioactive glassPDL stem cell lineResinRoot canal sealersSilicon

Identifiers

PMID34382118

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.