Evidence map›Paper›PMID 34382024›Full record

ArticleSTAR protocols2021

Protocol for setup and circadian analysis of inverted feeding in mice.

Haoran Xin, Rongfeng Huang, Meiyu Zhou, Xinyu Bao, Jianghui Chen, Fan Zeng, Xiaoqin Wan, Shifei Tong, Fang Deng, Min-Dian Li and 1 more

Abstract read
In one paragraph

Article in STAR protocols, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed.

  1. Article
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  5. Article
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  7. Article
  8. PPAR-PPAR research · 2022
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Haoran XinDepartment of Cardiology and the Center for Circadian Metabolism and Cardiovascular Disease, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing 400038, China.
Rongfeng HuangDepartment of Cardiology and the Center for Circadian Metabolism and Cardiovascular Disease, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing 400038, China.
Meiyu ZhouDepartment of Cardiology and the Center for Circadian Metabolism and Cardiovascular Disease, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing 400038, China.
Xinyu BaoDepartment of Cardiology and the Center for Circadian Metabolism and Cardiovascular Disease, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing 400038, China.
Jianghui ChenDepartment of Cardiology and the Center for Circadian Metabolism and Cardiovascular Disease, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing 400038, China.
Fan ZengDepartment of Cardiology and the Center for Circadian Metabolism and Cardiovascular Disease, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing 400038, China.
Xiaoqin WanDepartment of Cardiology and the Center for Circadian Metabolism and Cardiovascular Disease, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing 400038, China.
Shifei TongDepartment of Cardiology, the Third Affiliated Hospital of Chongqing Medical University, Chongqing 401120, China.
Fang DengDepartment of Pathophysiology, College of High Altitude Military Medicine, Key Laboratory of Extreme Environmental Medicine, Ministry of Education of China, and Key Laboratory of High Altitude Medicine, PLA, Army Medical University (Third Military Medical University), Chongqing 400038, China.
Min-Dian LiDepartment of Cardiology and the Center for Circadian Metabolism and Cardiovascular Disease, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing 400038, China.
Zhihui ZhangDepartment of Cardiology and the Center for Circadian Metabolism and Cardiovascular Disease, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing 400038, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Inverted feeding is a paradigm to study synchronization of circadian clocks by feeding rhythm in tissues more directly. Here, we provide a protocol for performing inverted feeding in mice and analyzing circadian rhythmicity in mouse tissues. We describe setting up inverted feeding and performing tissue dissection, followed by RNA extraction and gene expression analysis, and lastly R software-based analysis of circadian rhythmicity. This protocol can be combined with the use of CircaMetDB database for mechanistic studies of inverted feeding. For complete details on the use and execution of this protocol, please refer to Xin et al. (2021).

Indexed as

AnimalsCircadian ClocksCircadian RhythmComputational BiologyFeeding BehaviorMetabolismMicePeriod Circadian ProteinsPeriod Circadian ProteinsBehaviorBioinformaticsGene ExpressionMetabolismModel OrganismsMolecular BiologySystems biology

Identifiers

PMID34382024
PMCPMC8339291

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.