Evidence map›Paper›PMID 34382017›Full record

ArticleSTAR protocols2021

Measuring microtubule binding kinetics of membrane-bound kinesin motors using supported lipid bilayers.

Rui Jiang, William O Hancock

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.1field-weighted citation impact, top 61% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Rui JiangIntercollege Program in Integrative and Biomedical Physiology, Pennsylvania State University, University Park, PA 16802, USA.
William O HancockIntercollege Program in Integrative and Biomedical Physiology, Pennsylvania State University, University Park, PA 16802, USA.
Pennsylvania State University · US

Funding

Molecular mechanism of bidirectional transportR35GM139568 · NIGMS · PENNSYLVANIA STATE UNIVERSITY, THE · PI William Olaf Hancock · 2021 to 2026
$4.9M
Research Training in Physiological Adaptations to StressT32GM108563 · NIGMS · PENNSYLVANIA STATE UNIVERSITY, THE · PI CANTORNA, MARGHERITA T, KORZICK, DONNA HOPE · 2014 to 2023
$2.2M
Multimotor Mechanisms in Microtubule-based TransportR01GM121679 · NIGMS · PENNSYLVANIA STATE UNIVERSITY, THE · PI HANCOCK, WILLIAM OLAF · 2017 to 2020
$1.3M
NIGMS NIH HHS R01 GM121679NIGMS NIH HHS R35 GM139568NIGMS NIH HHS T32 GM108563
6 · The paper itself

Abstract

Membrane-bound cargos in cells are generally transported by multiple kinesin motors. Quantifying the bimolecular on-rate of motors for their microtubule track is important for understanding of multi-motor transport but is complicated by diffusion of the motors in the plane of the lipid bilayer. Here, we describe a method to measure the kinesin on-rate that uses a modified microtubule gliding assay performed on a supported lipid bilayer and detects motor binding by a local increase in fluorescence. For complete details on the use and execution of this protocol, please refer to Jiang et al. (2019).

Indexed as

Biological TransportDiffusionKinesinsKineticsLipid BilayersMembranesMicroscopy, FluorescenceMicrotubulesProtein BindingKinesinsLipid BilayersBiophysicsCell BiologyMicroscopyProtein BiochemistryProtein expression and purification

Identifiers

PMID34382017
PMCPMC8339383
OpenAlexW3153911904

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.