ArticleSTAR protocols2021
Protocol for the use of focused ion-beam milling to prepare crystalline lamellae for microcrystal electron diffraction (MicroED).
Article in STAR protocols, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Towards time-resolved MicroED grid preparation using mix-and-inject gas dynamic virtual nozzles.IUCrJ · 2026Article
- Membrane protein structure and dynamics probed by MicroED.Biochemical Society transactions · 2026Review
- Advances of 3D microcrystals electron diffraction for transmembrane protein structure determination.Biophysical journal · 2026Review
- Comprehensive microcrystal electron diffraction sample preparation for cryo-EM.Nature protocols · 2025Review
- VMXm - A sub-micron focus macromolecular crystallography beamline at Diamond Light Source.Journal of synchrotron radiation · 2024Article
- Article
- Reaching the potential of electron diffraction.Cell reports. Physical science · 2024Article
- Eliminating the missing cone challenge through innovative approaches.Journal of structural biology: X · 2024Article
- Applying 3D ED/MicroED workflows toward the next frontiers.Acta crystallographica. Section C, Structural chemistry · 2024Article
- Focused ion beam milling and MicroED structure determination of metal-organic framework crystals.Ultramicroscopy · 2024Article
- Advances and applications of microcrystal electron diffraction (MicroED).Current opinion in structural biology · 2024Review
- Structure determination of a DNA crystal by MicroED.Structure (London, England : 1993) · 2023Article
- A robust approach for MicroED sample preparation of lipidic cubic phase embedded membrane protein crystals.Nature communications · 2023Article
- Electron Diffraction of 3D Molecular Crystals.Chemical reviews · 2022Review
- Review
- Synthetic Biology Advanced Natural Product Discovery.Metabolites · 2021Review
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Authors and funding
2 authors.
Funding
Abstract
We present an in-depth protocol to reproducibly prepare crystalline lamellae from protein crystals for subsequent microcrystal electron diffraction (MicroED) experiments. This protocol covers typical soluble proteins and membrane proteins embedded in dense media. Following these steps will allow the user to prepare crystalline lamellae for protein structure determination by MicroED. For complete details on the use and execution of this protocol, please refer to Martynowycz et al. (2019a, 2020a).
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