ArticleiScience2021
Super-multiplex imaging of cellular dynamics and heterogeneity by integrated stimulated Raman and fluorescence microscopy.
Article in iScience, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Bridging the Resolution Gap in Recurrent Prostate Cancer Imaging: Quantum Dots as PSMA-Targeted Optical Probes.ACS omega · 2026Review
- Recent advances in Raman probes for multiplexed bioimaging.Inflammation and regeneration · 2025Review
- Illuminating life processes by vibrational probes.Nature methods · 2025Review
- Identification of tauopathy-associated lipid signatures in Alzheimer's disease mouse brain using label-free chemical imaging.Communications biology · 2024Article
- Similarity Metrics for Subcellular Analysis of FRET Microscopy Videos.The journal of physical chemistry. B · 2024Article
- Widely tunable fiber optical parametric oscillator synchronized with a Ti:sapphire laser for stimulated Raman scattering microscopy.Biomedical optics express · 2024Article
- The effect of sesamol on endogenous substances and oxidative stability of walnut oil.Frontiers in nutrition · 2024Article
- Bridging live-cell imaging and next-generation cancer treatment.Nature reviews. Cancer · 2023Review
- Deuterium- and Alkyne-Based Bioorthogonal Raman Probes for In Situ Quantitative Metabolic Imaging of Lipids within Plants.JACS Au · 2023Article
- Super-resolution vibrational imaging based on photoswitchable Raman probe.Science advances · 2023Article
- Analytical Techniques for Single-Cell Biochemical Assays of Lipids.Annual review of biomedical engineering · 2023Review
- Activatable Raman Probes Utilizing Enzyme-Induced Aggregate Formation for SelectiveJournal of the American Chemical Society · 2023Article
- 9-Cyano-10-telluriumpyronin Derivatives as Red-light-activatable Raman Probes.Chemistry, an Asian journal · 2023Article
- Imaging the uptake of deuterated methionine inFrontiers in chemistry · 2023Article
- Frequency modulation stimulated Raman scattering scheme for real-time background correction with a single light source.Biomedical optics express · 2023Article
- Super-multiplexing excitation spectral microscopy with multiple fluorescence bands.Biomedical optics express · 2022Article
- Miniaturized Chemical Tags for Optical Imaging.Angewandte Chemie (International ed. in English) · 2022Review
- Integrative metabolic flux analysis reveals an indispensable dimension of phenotypes.Current opinion in biotechnology · 2022Review
- Super-multiplexed vibrational probes: Being colorful makes a difference.Current opinion in chemical biology · 2022Review
- Direct visualization of general anesthetic propofol on neurons by stimulated Raman scattering microscopy.iScience · 2022Article
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
Observing multiple molecular species simultaneously with high spatiotemporal resolution is crucial for comprehensive understanding of complex, dynamic, and heterogeneous biological systems. The recently reported super-multiplex optical imaging breaks the "color barrier" of fluorescence to achieve multiplexing number over six in living systems, while its temporal resolution is limited to several minutes mainly by slow color tuning. Herein, we report integrated stimulated Raman and fluorescence microscopy with simultaneous multimodal color tunability at high speed, enabling super-multiplex imaging covering diverse molecular contrasts with temporal resolution of seconds. We highlight this technique by demonstrating super-multiplex time-lapse imaging and image-based cytometry of live cells to investigate the dynamics and cellular heterogeneity of eight intracellular components simultaneously. Our technique provides a powerful tool to elucidate spatiotemporal organization and interactions in biological systems.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.