ArticleScientific reports2021
Structural basis of the complementary activity of two ketosynthases in aryl polyene biosynthesis.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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9 citing papers in PubMed, 12 citations in OpenAlex.
- Community matters: stress tolerance and survival ofFrontiers in microbiology · 2026Article
- Structural and dynamic roles of tandem acyl carrier proteins in aryl polyene biosynthesis in carbapenem-resistant Acinetobacter baumannii.Protein science : a publication of the Protein Society · 2025Article
- Amphibian skin bacteria contain a wide repertoire of genes linked to their antifungal capacities.World journal of microbiology & biotechnology · 2025Article
- Genomic Insight intoPathogens (Basel, Switzerland) · 2025Article
- Acinetobacter corruptisaponis sp. nov., Isolated from a Spoiled Bath Lotion.Current microbiology · 2024Article
- A comprehensive comparative genomic analysis revealed that plant growth promoting traits are ubiquitous in strains ofFrontiers in microbiology · 2024Article
- Biosynthetic gene cluster signature profiles of pathogenic Gram-negative bacteria isolated from Egyptian clinical settings.Microbiology spectrum · 2023Article
- A comprehensive genomic analysis provides insights on the high environmental adaptability ofFrontiers in microbiology · 2023Article
- Enzymology of standalone elongating ketosynthases.Chemical science · 2022Review
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Aryl polyenes (APE) are one of the most widespread secondary metabolites among gram-negative bacteria. In Acinetobacter baumannii, strains belonging to the virulent global clone 2 (GC2) mostly contain APE biosynthesis genes; its relevance in elevated pathogenicity is of great interest. APE biosynthesis gene clusters harbor two ketosynthases (KSs): the heterodimeric KS-chain length factor complex, ApeO-ApeC, and the homodimeric ketoacyl-acyl carrier protein synthase I (FabB)-like KS, ApeR. The role of the two KSs in APE biosynthesis is unclear. We determined the crystal structures of the two KSs from a pathogenic A. baumannii strain. ApeO-ApeC and ApeR have similar cavity volumes; however, ApeR has a narrow cavity near the entrance. In vitro assay based on the absorption characteristics of polyene species indicated the generation of fully elongated polyene with only ApeO-ApeC, probably because of the funnel shaped active site cavity. However, adding ApeR to the reaction increases the throughput of APE biosynthesis. Mutagenesis at Tyr135 in the active site cavity of ApeR reduces the activity significantly, which suggests that the stacking of the aryl group between Tyr135 and Phe202 is important for substrate recognition. Therefore, the two KSs function complementarily in the generation of APE to enhance its production.
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