ArticleScience advances2021
Stochastic gene expression drives mesophyll protoplast regeneration.
Article in Science advances, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
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Who cites it
62 citing papers in PubMed.
- Molecular understanding of plant pollination, fertilization, seed development and protoplast regeneration.Science China. Life sciences · 2026Review
- Auxin promotes robust founder cell specification during Arabidopsis lateral root initiation.Genetics · 2026Article
- Transcriptomic and proteomic insights into the molecular mechanism underlying cell wall biosynthesis in Moso bamboo (Phyllostachys edulis).Plant cell reports · 2026Article
- Cell-specific dedifferentiation inbioRxiv : the preprint server for biology · 2026Article
- Protoplasts Isolation and Transient Transformation System Optimization for Poplar 84K (Biology · 2026Article
- A single-cell screening platform accelerates functional genetics in plants.Nature biotechnology · 2026Article
- A single-cell transcriptome atlas reveals the transcriptionally earliest state in rice callus.BMC plant biology · 2026Article
- Dedifferentiation of Plant Cells: A Term Covering Multiple Pathways?Plants (Basel, Switzerland) · 2026Article
- Comprehensive transcriptomic and functional characterization of protoplast regeneration in Angelica gigas Nakai.BMC plant biology · 2026Article
- Single-cell transcriptomics reveals cellular and genetic mechanisms of alpine adaptation inFrontiers in plant science · 2026Article
- ZmHSCF1 enhances Agrobacterium-mediated transformation frequency in commercial maize inbred lines by promoting embryogenic callus proliferation.Plant communications · 2025Article
- Building resilience by cultivating difference: A role for noise in development.Current opinion in plant biology · 2025Review
- Woody Plant Transformation: Current Status, Challenges, and Future Perspectives.Plants (Basel, Switzerland) · 2025Review
- Rational design of induced regeneration via somatic embryogenesis in the absence of exogenous phytohormones.The Plant cell · 2025Article
- Genome assembly of two allotetraploid cotton germplasms reveals mechanisms of somatic embryogenesis and enables precise genome editing.Nature genetics · 2025Article
- Plant genetic transformation: achievements, current status and future prospects.Plant biotechnology journal · 2025Review
- Stochastic gene expression in auxin signaling in the floral meristem of Arabidopsis thaliana.Nature communications · 2025Article
- Dual and spatially resolved drought responses in the Arabidopsis leaf mesophyll revealed by single-cell transcriptomics.The New phytologist · 2025Article
- Global landscape of protein phosphorylation during plant regeneration initiation in cotton (Gossypium hirsutum L.).BMC biology · 2025Article
- Demethylating drugs alter protoplast development, regeneration, and the genome stability of protoplast-derived regenerants of cabbage.BMC plant biology · 2025Article
2 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cell pluripotency is fundamental to biology. It has long been known that differentiated somatic plant cells may reacquire pluripotency, but the underlying mechanism remains elusive. In many plant species, a single isolated mesophyll protoplast may regenerate into an entire plant, which is widely used in gene transformation. Here, we identified two transcription factors whose ectopic activation promotes protoplast regeneration. Furthermore, we found that their expression was induced by protoplast isolation but at a very low frequency. Using live imaging and single-cell transcriptomics, we show that isolating protoplasts induces enhanced expression variation at the genome level. Isolating protoplasts also leads to genome-wide increases in chromatin accessibility, which promotes stochastic activation of gene expression and enhances protoplast regeneration. We propose that transcriptome chaos with increased expression variability among cells creates a cellular-level evolutionary driver selecting for regenerating cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.