Evidence map›Paper›PMID 34380522›Full record

ArticleJournal of nanobiotechnology2021

Intranasal immunization with O-2'-Hydroxypropyl trimethyl ammonium chloride chitosan nanoparticles loaded with Newcastle disease virus DNA vaccine enhances mucosal immune response in chickens.

Kai Zhao, Beini Sun, Ci Shi, Yanwei Sun, Zheng Jin, Gaowei Hu

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of nanobiotechnology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
1.5field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 30 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Kai ZhaoInstitute of Nanobiomaterials and Immunology, School of Life Science, Taizhou University, Taizhou, 318000, China. zybin395@126.com.
Beini SunKey Laboratory of Microbiology, College of Heilongjiang Province, School of Life Science, Heilongjiang University, Harbin, 150080, China.
Ci ShiKey Laboratory of Microbiology, College of Heilongjiang Province, School of Life Science, Heilongjiang University, Harbin, 150080, China.
Yanwei SunKey Laboratory of Microbiology, College of Heilongjiang Province, School of Life Science, Heilongjiang University, Harbin, 150080, China.
Zheng JinInstitute of Nanobiomaterials and Immunology, School of Life Science, Taizhou University, Taizhou, 318000, China.
Gaowei HuInstitute of Nanobiomaterials and Immunology, School of Life Science, Taizhou University, Taizhou, 318000, China.
Heilongjiang University · CNTaizhou University · CN

Funding

National Natural Science Foundation of China 31771000Young Eagle Plan for Cultivation Special Project of Scientific and Technological Enterprise in Harbin 2020CYJBCG0306Zhejiang Provincial Key R&D Program of China 2021C02049
6 · The paper itself

Abstract

backgroundThere has been a great interest in developing strategies for enhancing antigen delivery to the mucosal immune system as well as identifying mucosal active immunostimulating agents. To elevate the potential of O-2'-Hydroxypropyl trimethyl ammonium chloride chitosan (O-2'-HACC) as an adjuvant and mucosal immune delivery carrier for DNA vaccine, we prepared the O-2'-HACC loaded with Newcastle disease virus (NDV) F gene plasmid DNA and C3d6 molecular adjuvant (O-2'-HACC/pFDNA microparticles).

resultsThe O-2'-HACC/pFDNA exhibited a regular spherical morphology with a particle size of 202.3 ± 0.52 nm, a zeta potential of 50.8 ± 8.21 mV, encapsulation efficiency of 90.74 ± 1.10%, and a loading capacity of 49.84 ± 1.20%. The plasmid DNA could be sustainably released from the O-2'-HACC/pFDNA after an initial burst release. Intranasal vaccination of chickens immunized with O-2'-HACC/pFDNA not only induced higher anti-NDV IgG and sIgA antibody titers but also significantly promoted lymphocyte proliferation and produced higher levels of IL-2, IL-4, IFN-γ, CD4+, and CD8 + T lymphocytes compared with the NDV commercial live attenuated vaccine. Intranasal delivery of the O-2'-HACC/pFDNA enhanced humoral, cellular, and mucosal immune responses and protected chickens from the infection of highly virulent NDV compared with the intramuscular delivery.

conclusionsCollectively, our findings indicated that the O-2'-HACC could be used as a vaccine adjuvant and delivery system for mucosal immunity and have an immense application promise.

Indexed as

VaccinationAdjuvants, VaccineAdministration, IntranasalAmmonium ChlorideAnimalsChickensChitosanImmunity, MucosalImmunizationNewcastle DiseaseNewcastle disease virusParticle SizeVaccines, DNAViral VaccinesAdjuvants, VaccineAmmonium ChlorideChitosanVaccines, DNAViral VaccinesDNA vaccineIntranasal deliveryMucosal immunityNewcastle disease virusO-2ʹ-Hydroxypropyl trimethyl ammonium chloride chitosan microparticles

Identifiers

PMID34380522
PMCPMC8359106
OpenAlexW3171668163

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.