Evidence map›Paper›PMID 34380030›Full record

ArticleCell reports2021

HIV-1 Nef interacts with the cyclin K/CDK13 complex to antagonize SERINC5 for optimal viral infectivity.

Qingqing Chai, Sunan Li, Morgan K Collins, Rongrong Li, Iqbal Ahmad, Silas F Johnson, Dylan A Frabutt, Zhichang Yang, Xiaojing Shen, Liangliang Sun and 4 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
1.4field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 15 citations in OpenAlex.

  1. Manipulation of the cell cycle by HIV-1.Biochemical Society transactions · 2026
    Review
  2. Review
  3. Article
  4. Article
  5. Article
  6. Review
  7. SERINC5 counters retroviruses and non-retroviruses.Frontiers in cellular and infection microbiology · 2024
    Review
  8. HIV-1 subtypes maintain distinctive physicochemical signatures in Nef domains associated with immunoregulation.Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases · 2023
    Article
  9. HIV-1 restriction by SERINC5.Medical microbiology and immunology · 2023
    Review
  10. Article
  11. Article
  12. Article
  13. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 5 institutions in 2 countries.

Qingqing ChaiDepartment of Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI, USA.
Sunan LiHarbin Veterinary Research Institute, CAAS-Michigan State University Joint Laboratory of Innate Immunity, State Key Laboratory of Veterinary Biotechnology, Chinese Academy of Agricultural Sciences, Harbin, China.
Morgan K CollinsDepartment of Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI, USA.
Rongrong LiHarbin Veterinary Research Institute, CAAS-Michigan State University Joint Laboratory of Innate Immunity, State Key Laboratory of Veterinary Biotechnology, Chinese Academy of Agricultural Sciences, Harbin, China.
Iqbal AhmadHarbin Veterinary Research Institute, CAAS-Michigan State University Joint Laboratory of Innate Immunity, State Key Laboratory of Veterinary Biotechnology, Chinese Academy of Agricultural Sciences, Harbin, China.
Silas F JohnsonDepartment of Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI, USA; Department of Biology, Hillsdale College, Hillsdale, MI, USA.
Dylan A FrabuttDepartment of Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI, USA.
Zhichang YangDepartment of Chemistry, Michigan State University, East Lansing, MI, USA.
Xiaojing ShenDepartment of Chemistry, Michigan State University, East Lansing, MI, USA.
Liangliang SunDepartment of Chemistry, Michigan State University, East Lansing, MI, USA.
Jian HuDepartment of Chemistry, Michigan State University, East Lansing, MI, USA; Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, MI, USA.
Judd F HultquistDivision of Infectious Diseases, Northwestern University Feinberg School of Medicine, Chicago, IL, USA.
B Matija PeterlinDepartment of Medicine, University of California, San Francisco, San Francisco, CA, USA; Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA, USA.
Yong-Hui ZhengHarbin Veterinary Research Institute, CAAS-Michigan State University Joint Laboratory of Innate Immunity, State Key Laboratory of Veterinary Biotechnology, Chinese Academy of Agricultural Sciences, Harbin, China; Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI, USA. Electronic address: zhengyo@msu.edu.
Michigan State University · USChinese Academy of Agricultural Sciences · CNHillsdale College · USNorthwestern University · USUniversity of California, San Francisco · US

Funding

X-ray Screening and Rapid Structure DeterminationP50GM082250 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI KROGAN, NEVAN J · 2007 to 2018
$48.8M
TAT TransactivationR01AI049104 · NIAID · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI PETERLIN, BORIS MATIJA · 2001 to 2015
$5.3M
TAT TransactivationR01AI125104 · NIAID · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI PETERLIN, BORIS MATIJA · 2016 to 2020
$2.0M
Deciphering the Role of CPSF6 in HIV InfectionR01AI165236 · NIAID · NORTHWESTERN UNIVERSITY AT CHICAGO · PI HULTQUIST, JUDD F · 2021 to 2025
$2.0M
The role of SERINC5 in HIV-1 replicationR01AI145504 · NIAID · UNIVERSITY OF ILLINOIS AT CHICAGO · PI ZHENG, YONG-HUI · 2019 to 2023
$1.5M
NIAID NIH HHS R01 AI049104NIAID NIH HHS R01 AI125104NIAID NIH HHS R01 AI145504NIAID NIH HHS R01 AI165236NIGMS NIH HHS P50 GM082250
6 · The paper itself

Abstract

HIV-1-negative factor (Nef) protein antagonizes serine incorporator 5 (SERINC5) by redirecting this potent restriction factor to the endosomes and lysosomes for degradation. However, the precise mechanism remains unclear. Using affinity purification/mass spectrometry, we identify cyclin K (CycK) and cyclin-dependent kinase 13 (CDK13) as a Nef-associated kinase complex. CycK/CDK13 phosphorylates the serine at position 360 (S360) in SERINC5, which is required for Nef downregulation of SERINC5 from the cell surface and its counteractivity of the SERINC5 antiviral activity. To understand the role of S360 phosphorylation, we generate chimeric proteins between CD8 and SERINC5 to study their response to Nef. Nef not only downregulates but, importantly, also binds to this chimera in an S360-dependent manner. Thus, S360 phosphorylation increases interactions between Nef and SERINC5 and initiates the destruction of SERINC5 by the endocytic machinery.

Indexed as

Amino Acid SequenceCDC2 Protein KinaseCyclinsDown-RegulationHEK293 CellsHIV-1HIV InfectionsHumansJurkat CellsMass SpectrometryMembrane Proteinsnef Gene Products, Human Immunodeficiency VirusPeptidesPhosphorylationPhosphoserineProtein BindingCCNK protein, humanCDC2 Protein KinaseCDK13 protein, humanCyclinsMembrane Proteinsnef Gene Products, Human Immunodeficiency VirusPeptidesPhosphoserineRecombinant Fusion ProteinsSERINC5 protein, humanAP-2CDKcyclinendocytosisHIV-1intrinsic immunityNefrestriction factorsSERINC5serine phosphorylation

Identifiers

PMID34380030
PMCPMC8385645
OpenAlexW3191226416

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.