ArticleLab on a chip2021
Counting of enzymatically amplified affinity reactions in hydrogel particle-templated drops.
Article in Lab on a chip, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
9 citing papers in PubMed.
- Tunable Picoliter-Scale Dropicle Formation Using Amphiphilic Microparticles with Patterned Hydrophilic Patches.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Lab on a Particle Technologies.Analytical chemistry · 2024Review
- Fluid dynamics alters liquid-liquid phase separation in confined aqueous two-phase systems.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Digital Cascade Assays for ADP- or ATP-Producing Enzymes Using a Femtoliter Reactor Array Device.ACS sensors · 2023Article
- Article
- Hydrogel-Based Multi-enzymatic System for Biosynthesis.Advances in biochemical engineering/biotechnology · 2023Review
- Ultrasensitive Single Extracellular Vesicle Detection Using High Throughput Droplet Digital Enzyme-Linked Immunosorbent Assay.Nano letters · 2022Article
- Recent Advances in Polymer Additive Engineering for Diagnostic and Therapeutic Hydrogels.International journal of molecular sciences · 2022Review
- Pico-washing: simultaneous liquid addition and removal for continuous-flow washing of microdroplets.Microsystems & nanoengineering · 2022Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Counting of numerous compartmentalized enzymatic reactions underlies quantitative and high sensitivity immunodiagnostic assays. However, digital enzyme-linked immunosorbent assays (ELISA) require specialized instruments which have slowed adoption in research and clinical labs. We present a lab-on-a-particle solution to digital counting of thousands of single enzymatic reactions. Hydrogel particles are used to bind enzymes and template the formation of droplets that compartmentalize reactions with simple pipetting steps. These hydrogel particles can be made at a high throughput, stored, and used during the assay to create ∼500 000 compartments within 2 minutes. These particles can also be dried and rehydrated with sample, amplifying the sensitivity of the assay by driving affinity interactions on the hydrogel surface. We demonstrate digital counting of β-galactosidase enzyme at a femtomolar detection limit with a dynamic range of 3 orders of magnitude using standard benchtop equipment and experiment techniques. This approach can faciliate the development of digital ELISAs with reduced need for specialized microfluidic devices, instruments, or imaging systems.
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Registered trials
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