ArticleJournal of biomedical optics2021
Hemoglobin quantification in red blood cells via dry mass mapping based on UV absorption.
Article in Journal of biomedical optics, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Quantification and diagnostic relevance of blood and heme-mediated inhibition of prion detection by RT-QuIC.Journal of clinical microbiology · 2026Article
- Nondestructive, High-Resolution T Cell Characterization and Subtyping via Deep-UV Microscopy.BME frontiers · 2026Article
- Label-free deep UV microscopy in oral cytology: a step towards stain-free diagnostics.Biomedical optics express · 2025Article
- Rapid, Point-of-Care Bone Marrow Aspirate Adequacy Assessment Via Deep Ultraviolet Microscopy.Laboratory investigation; a journal of technical methods and pathology · 2025Article
- HOPS/CORVET tethering complexes are critical for endocytosis and protein trafficking to invasion related organelles in malaria parasites.PLoS pathogens · 2025Article
- Development of Engineered Microparticles for Investigating Enzymatic Degradation of Proteins and Peptides within Phagosomes.ACS applied materials & interfaces · 2025Article
- RBC-GEM: A genome-scale metabolic model for systems biology of the human red blood cell.PLoS computational biology · 2025Article
- Computational Prediction of Tissue Iron Dynamics in Iron Deficiency Anemia Following Intravenous Ferric Carboxymaltose Therapy.International journal of nanomedicine · 2025Article
- Quantifying UV-induced photodamage for longitudinal live-cell imaging applications of deep-UV microscopy.Biomedical optics express · 2025Article
- Biothermodynamics of Hemoglobin and Red Blood Cells: Analysis of Structure and Evolution of Hemoglobin and Red Blood Cells, Based on Molecular and Empirical Formulas, Biosynthesis Reactions, and Thermodynamic Properties of Formation and Biosynthesis.Journal of molecular evolution · 2024Article
- Compact and low-cost deep-ultraviolet microscope system for label-free molecular imaging and point-of-care hematological analysis.Biomedical optics express · 2023Article
- X-ray crystallography and sickle cell disease drug discovery-a tribute to Donald Abraham.Frontiers in molecular biosciences · 2023Review
- Label-free hematology analysis method based on defocusing phase-contrast imaging under illumination of 415 nm light.Biomedical optics express · 2022Article
- Virtual Staining, Segmentation, and Classification of Blood Smears for Label-Free Hematology Analysis.BME frontiers · 2022Article
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3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
significanceThe morphological properties and hemoglobin (Hb) content of red blood cells (RBCs) are essential biomarkers to diagnose or monitor various types of hematological disorders. Label-free mass mapping approaches enable accurate Hb quantification from individual cells, serving as promising alternatives to conventional hematology analyzers. Deep ultraviolet (UV) microscopy is one such technique that allows high-resolution, molecular imaging, and absorption-based mass mapping.
aimTo compare UV absorption-based mass mapping at four UV wavelengths and understand variations across wavelengths and any assumptions necessary for accurate Hb quantification. APPROACH: Whole blood smears are imaged with a multispectral UV microscopy system, and the RBCs' dry masses are computed. This approach is compared to quantitative phase imaging-based mass mapping using data from an interferometric UV imaging system.
resultsConsistent Hb mass and mean corpuscular Hb values are obtained at all wavelengths, with the precision of the single-cell mass measurements being nearly identical at 220, 260, and 280 nm but slightly lower at 300 nm.
conclusionsA full hematological analysis (including white blood cell identification and characterization, and Hb quantification) may be achieved using a single UV illumination wavelength, thereby improving the speed and cost-effectiveness.
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