Evidence map›Paper›PMID 34378123›Full record

ArticleEndocrine2022

Analysis of serum microRNA in exosomal vehicles of papillary thyroid cancer.

Francesca Capriglione, Antonella Verrienti, Marilena Celano, Valentina Maggisano, Marialuisa Sponziello, Valeria Pecce, Agnese Gagliardi, Laura Giacomelli, Valerio Aceti, Cosimo Durante and 2 more

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In one paragraph

Article in Endocrine, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
22citing papers in PubMed, 2 pooled it
1.2field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

22 citing papers in PubMed, 2 syntheses or guidelines pooled it, 22 citations in OpenAlex.

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  19. CTC, ctDNA, and Exosome in Thyroid Cancers: A Review.International journal of molecular sciences · 2023
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 2 institutions in 1 country.

Francesca Capriglione *Department of Health Sciences, University "Magna Graecia" of Catanzaro, 88100, Catanzaro, Italy.
Antonella Verrienti *Department of translational and precision medicine, "Sapienza" University of Rome, 00161, Rome, Italy.
Marilena CelanoDepartment of Health Sciences, University "Magna Graecia" of Catanzaro, 88100, Catanzaro, Italy.
Valentina MaggisanoDepartment of Health Sciences, University "Magna Graecia" of Catanzaro, 88100, Catanzaro, Italy.
Marialuisa SponzielloDepartment of translational and precision medicine, "Sapienza" University of Rome, 00161, Rome, Italy.
Valeria PecceDepartment of translational and precision medicine, "Sapienza" University of Rome, 00161, Rome, Italy.
Agnese GagliardiDepartment of Health Sciences, University "Magna Graecia" of Catanzaro, 88100, Catanzaro, Italy.
Laura GiacomelliDepartment of Surgical Sciences, "Sapienza" University of Rome, 00161, Rome, Italy.
Valerio AcetiDepartment of translational and precision medicine, "Sapienza" University of Rome, 00161, Rome, Italy.
Cosimo DuranteDepartment of translational and precision medicine, "Sapienza" University of Rome, 00161, Rome, Italy.
Stefania BulottaDepartment of Health Sciences, University "Magna Graecia" of Catanzaro, 88100, Catanzaro, Italy.
Diego RussoDepartment of Health Sciences, University "Magna Graecia" of Catanzaro, 88100, Catanzaro, Italy. d.russo@unicz.it.ORCID http://orcid.org/0000-0001-7559-1595
Magna Graecia University · ITSapienza University of Rome · IT

Funding

miur-prin PRIN2017EKMFTN_003
6 · The paper itself

Abstract

purposeIn this study, we investigated the profile of microRNAs (miRNAs) contained in exosomes secreted in the serum of patients with papillary thyroid cancer (PTC).

methodsExosome were isolated by adding ExoQuick Exosome Precipitation Solution. Dynamic light scattering (DLS) and western blotting analysis were used to ensure the quality of exosomes. The expression levels of miRNAs were investigated using custom-designed TaqMan Advanced miRNA Array Cards in the screening cohort and using specific TaqMan Advanced MicroRNA Assays in the validation cohort.

resultsWe identified miR24-3p, miR146a-5p, miR181a-5p and miR382-5p with different expression levels in two different series of 56 and 58 PTC patients as compared with healthy controls. Significant differences in the expression of three PTC exosomal miRNAs, depending on the presence of lymph node metastasis, were detected in only one PTC series. When comparing the expression levels of some PTC-specific exosomal miRNAs with those of the same miRNAs circulating free of any encapsulation, we found a significant correlation for only miR24-3p, suggesting that only select miRNAs are secreted in exosomes.

conclusionsOur findings demonstrate that four miRNAs are differently secreted in the exosomes of PTC patients, whereas no conclusive results were found to characterize PTCs with lymph node metastasis, suggesting caution in the use of circulating exosomal miRNA expression levels as lymph node metastasis biomarkers. Further investigation into the mechanisms governing miRNA secretion in tumor cells are required.

Indexed as

Circulating MicroRNAExosomesMicroRNAsThyroid NeoplasmsHumansThyroid Cancer, PapillaryCirculating MicroRNAMicroRNAsMIRN24 microRNA, humanExtracellular vesiclesGene expressionMicroRNAThyroid cancer exosomes

Identifiers

PMID34378123
OpenAlexW3190687464

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.