ArticleTurkish journal of biology = Turk biyoloji dergisi2021
Reconstitution of Pol II (G) responsive form of the human Mediator complex.
Article in Turkish journal of biology = Turk biyoloji dergisi, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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2 citing papers in PubMed, 5 citations in OpenAlex.
- Biochemical and epigenomic dissection of TFIIE function reveals gene-selective requirement in human transcription.Scientific reports · 2026Article
- Application and research progress of MultiBac: A review.Medicine · 2024Review
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1 author at 1 institution in 2 countries.
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Abstract
RNA polymerase II (Pol II) is a 12 subunit protein complex from yeast to human that is required for gene expression. Gdown1 containing Pol II [Pol II (G)] is a special form of Pol II that is catalytically inactive and heavily depends on the 30-subunit Mediator complex for its activator and basal dependent function in vitro. Here we report for the first time, the identification and the generation of a 15-subunit human Mediator complex via the novel multibac baculovirus expression system that is fully responsive to Pol II (G). Our results show complete recovery of Pol II (G) dependent transcription both with full 30-subunit Mediator and also with 15-subunit recombinant Mediator that we synthesized. Moreover, we also show that the recombinant Mediator interacts with Pol II (G) as well. These results enlighten us towards understanding how a certain population of Pol II that is involved in selected gene regulation is activated by Mediator complex.
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