ArticleScientific reports2021
Clinical validation of optimised RT-LAMP for the diagnosis of SARS-CoV-2 infection.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 28 citations in OpenAlex.
- Article
- Diagnostic value comparative analysis of the commercial kits for the detection of SARS-CoV-2 in clinical samples: a systematic review and meta-analysis.Iranian journal of microbiology · 2025Review
- Rapid, Economical Detection ofACS omega · 2025Article
- Rapid quantitative PCR equipment using photothermal conversion of Au nanoshell.Scientific reports · 2024Article
- Quantitative mRNA expression measurement at home.Scientific reports · 2024Article
- LoCKAmp: lab-on-PCB technology for <3 minute virus genetic detection.Lab on a chip · 2023Article
- Clinical Validation of a Colorimetric Loop-Mediated Isothermal Amplification Using a Portable Device for the Rapid Detection of SARS-CoV-2.Diagnostics (Basel, Switzerland) · 2023Article
- Vivid COVID-19 LAMP is an ultrasensitive, quadruplexed test using LNA-modified primers and a zinc ion and 5-Br-PAPS colorimetric detection system.Communications biology · 2023Article
- Rapid and Flexible RT-qPCR Surveillance Platforms To Detect SARS-CoV-2 Mutations.Microbiology spectrum · 2023Article
- Starting from scratch: Step-by-step development of diagnostic tests for SARS-CoV-2 detection by RT-LAMP.PloS one · 2023Article
- VIDIIA Hunter diagnostic platform: a low-cost, smartphone connected, artificial intelligence-assisted COVID-19 rapid diagnostics approved for medical use in the UK.Frontiers in molecular biosciences · 2023Article
- A quantitative RT-qLAMP for the detection of SARS-CoV-2 and human gene in clinical application.Microbial biotechnology · 2022Article
- Loop-mediated isothermal amplification-based electrochemical sensor for detecting SARS-CoV-2 in wastewater samples.Journal of environmental chemical engineering · 2022Article
- Visual Detection of COVID-19 from Materials Aspect.Advanced fiber materials · 2022Review
- A semi-automated, isolation-free, high-throughput SARS-CoV-2 reverse transcriptase (RT) loop-mediated isothermal amplification (LAMP) test.Scientific reports · 2021Article
- Point-of-Care Diagnostic Tools for Surveillance of SARS-CoV-2 Infections.Frontiers in public health · 2021Review
Corrections and comments
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Authors and funding
22 authors at 6 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have optimised a reverse transcription-loop-mediated isothermal amplification (RT-LAMP) assay for the detection of SARS-CoV-2 from extracted RNA for clinical application. We improved the stability and reliability of the RT-LAMP assay by the addition of a temperature-dependent switch oligonucleotide to reduce self- or off-target amplification. We then developed freeze-dried master mix for single step RT-LAMP reaction, simplifying the operation for end users and improving long-term storage and transportation. The assay can detect as low as 13 copies of SARS-CoV2 RNA per reaction (25-μL). Cross reactivity with other human coronaviruses was not observed. We have applied the new RT-LAMP assay for testing clinical extracted RNA samples extracted from swabs of 72 patients in the UK and 126 samples from Greece and demonstrated the overall sensitivity of 90.2% (95% CI 83.8-94.7%) and specificity of 92.4% (95% CI 83.2-97.5%). Among 115 positive samples which Ct values were less than 34, the RT-LAMP assay was able to detect 110 of them with 95.6% sensitivity. The specificity was 100% when RNA elution used RNase-free water. The outcome of RT-LAMP can be reported by both colorimetric detection and quantifiable fluorescent reading. Objective measures with a digitized reading data flow would allow for the sharing of results for local or national surveillance.
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