Evidence map›Paper›PMID 34375585›Full record

ArticleMolecular cell2021

Spatiotemporal coordination of transcription preinitiation complex assembly in live cells.

Vu Q Nguyen, Anand Ranjan, Sheng Liu, Xiaona Tang, Yick Hin Ling, Jan Wisniewski, Gaku Mizuguchi, Kai Yu Li, Vivian Jou, Qinsi Zheng and 3 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular cell, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 83 papers.

0numbers the graph read from it
0cells of the map it votes in
83citing papers in PubMed
7.5field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

83 citing papers in PubMed, 125 citations in OpenAlex.

  1. Review
  2. The molecular basis of transcription initiation by RNA polymerase II.Nature reviews. Molecular cell biology · 2026
    Review
  3. Article
  4. Review
  5. Article
  6. Article
  7. Review
  8. Article
  9. Review
  10. Article
  11. What makes genes burst.Trends in cell biology · 2026
    Review
  12. Article
  13. Article
  14. Article
  15. Article
  16. Article
  17. Review
  18. Article
  19. Article
  20. Review

23 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

13 authors at 3 institutions in 1 country.

Vu Q NguyenDepartment of Biology, Johns Hopkins University, Baltimore, MD 21218, USA.
Anand RanjanDepartment of Biology, Johns Hopkins University, Baltimore, MD 21218, USA.
Sheng LiuDepartment of Biology, Johns Hopkins University, Baltimore, MD 21218, USA.
Xiaona TangDepartment of Biology, Johns Hopkins University, Baltimore, MD 21218, USA.
Yick Hin LingDepartment of Biology, Johns Hopkins University, Baltimore, MD 21218, USA.
Jan WisniewskiJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA; Experimental Immunology Branch, National Cancer Institute, Bethesda, MD 20892, USA.
Gaku MizuguchiDepartment of Biology, Johns Hopkins University, Baltimore, MD 21218, USA.
Kai Yu LiDepartment of Biology, Johns Hopkins University, Baltimore, MD 21218, USA.
Vivian JouDepartment of Biology, Johns Hopkins University, Baltimore, MD 21218, USA.
Qinsi ZhengJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA.
Luke D LavisJanelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA.
Timothée LionnetInstitute of Systems Genetics, Langone Medical Center, New York University, New York, NY 10016, USA.
Carl WuDepartment of Biology, Johns Hopkins University, Baltimore, MD 21218, USA; Department of Molecular Biology and Genetics, Johns Hopkins School of Medicine, Baltimore, MD 21287, USA. Electronic address: wuc@jhu.edu.
Johns Hopkins University · USHoward Hughes Medical Institute · USNYU Langone Health · US

Funding

Dynamic association of transcription initiation proteins with chromatin at single-molecule resolution in living yeastR01GM132290 · NIGMS · JOHNS HOPKINS UNIVERSITY · PI WU, CARL · 2019 to 2022
$1.8M
Howard Hughes Medical InstituteNIGMS NIH HHS R01 GM132290
6 · The paper itself

Abstract

Transcription initiation by RNA polymerase II (RNA Pol II) requires preinitiation complex (PIC) assembly at gene promoters. In the dynamic nucleus, where thousands of promoters are broadly distributed in chromatin, it is unclear how multiple individual components converge on any target to establish the PIC. Here we use live-cell, single-molecule tracking in S. cerevisiae to visualize constrained exploration of the nucleoplasm by PIC components and Mediator's key role in guiding this process. On chromatin, TFIID/TATA-binding protein (TBP), Mediator, and RNA Pol II instruct assembly of a short-lived PIC, which occurs infrequently but efficiently within a few seconds on average. Moreover, PIC exclusion by nucleosome encroachment underscores regulated promoter accessibility by chromatin remodeling. Thus, coordinated nuclear exploration and recruitment to accessible targets underlies dynamic PIC establishment in yeast. Our study provides a global spatiotemporal model for transcription initiation in live cells.

Indexed as

ChromatinChromatin Assembly and DisassemblyMediator ComplexNucleosomesPromoter Regions, GeneticProtein BindingRNA Polymerase IISaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsSpatio-Temporal AnalysisTATA-Box Binding ProteinTranscription Factor TFIIDTranscription, GeneticTranscription Initiation, GeneticChromatinMediator ComplexNucleosomesRNA Polymerase IISaccharomyces cerevisiae ProteinsTATA-Box Binding ProteinTranscription Factor TFIIDlive-cell imagingMediatorsingle-molecule trackingsubdiffusionTranscription initiation

Identifiers

PMID34375585
PMCPMC8420877
OpenAlexW3189598002

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.