ArticlePloS one2021
Utilization of somatic fusion techniques for the development of HLB tolerant breeding resources employing the Australian finger lime (Citrus australasica).
Article in PloS one, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 32 citations in OpenAlex.
- Inward Rectifier Potassium (Kir) Channel Inhibitors Protect Citrus from the Asian Citrus Psyllid by Inducing Toxicity and Inhibition of Feeding.Journal of agricultural and food chemistry · 2026Article
- Transcriptional regulation of thorn tip sclerification in plants.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Differential Nutrient Contents and Free Amino Acid Levels in Asymptomatic and Symptomatic Leaves of Huanglongbing-Affected Grapefruit Trees.Plants (Basel, Switzerland) · 2025Article
- Unlocking the Sublime: A Review of Native AustralianFoods (Basel, Switzerland) · 2025Review
- Transcriptomic and biochemical analysis of pummelo x finger lime hybrids in response to Huanglongbing (HLB).BMC plant biology · 2025Article
- Novel citrus hybrids incorporating Australian lime genetics: development of HLB-tolerant citrus rootstocks and physiological changes in 'Valencia' sweet orange scions.Frontiers in plant science · 2025Article
- Modern Technologies Provide New Opportunities for Somatic Hybridization in the Breeding of Woody Plants.Plants (Basel, Switzerland) · 2024Review
- Antibacterial Activity of Ethanol Extract from Australian Finger Lime.Foods (Basel, Switzerland) · 2024Article
- DeepFrontiers in plant science · 2024Article
- Comparative Analysis of Physicochemical Properties and Storability of a New Citrus Variety, Yellowball, and Its Parent.Plants (Basel, Switzerland) · 2023Article
- Overexpression of the salicylic acid binding protein 2 (SABP2) from tobacco enhances tolerance against Huanglongbing in transgenic citrus.Plant cell reports · 2022Article
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Australian finger lime is a unique citrus species that has gained importance due to its unique fruit characteristics and perceived tolerance to Huanglongbing (HLB), an often-fatal disease of citrus trees. In this study, we developed allotetraploid finger lime hybrids and cybrids by utilizing somatic cell fusion techniques to fuse diploid 'OLL8' sweet orange or 'Page' tangelo callus-derived protoplasts with finger lime (FL) mesophyll-derived protoplasts. Six somatic fusions were regenerated from the 'OLL8' + FL fusion, while three putative cybrids were regenerated from the 'Page' + FL fusion. Ploidy levels and nuclear-expressed sequence tag derived simple sequence repeat (EST-SSR) markers confirmed the somatic hybrid production, and mitochondrial DNA primer sets confirmed the cybrid nature. Several trees produced by the somatic fusion remained HLB negative even after 6 years of growth in an HLB-endemic environment. Pathogenesis related (PR) and other genes that are often upregulated in HLB-tolerant trees were also upregulated in our somatic fusions. These newly developed somatic fusions and cybrids could potentially be used as breeding parents to develop the next generation of improved HLB-tolerant rootstocks and scions.
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