ArticleReproductive sciences (Thousand Oaks, Calif.)2022
The Differential Metabolomes in Cumulus and Mural Granulosa Cells from Human Preovulatory Follicles.
Article in Reproductive sciences (Thousand Oaks, Calif.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 1 of them a synthesis that pooled it.
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Who cites it
21 citing papers in PubMed, 1 synthesis or guideline pooled it, 27 citations in OpenAlex.
- Unravelling the role of HAS2, GREM1, and PTGS2 gene expression in cumulus cells: implications for human oocyte development competency - a systematic review and integrated bioinformatic analysis.Frontiers in endocrinology · 2024Pooled it
- Molecular interplay between sperm and oocyte: a narrative review.Human reproduction update · 2026Review
- Metabolomic profiling delineates the role of adenosine in oocyte quality and embryonic development.Cell death & disease · 2026Article
- Integrated Multi-Omics Analysis Reveals the Role of the Gut Microbiota-Metabolite-Endocrine Axis in Post-Weaning Estrus Recovery in Tibetan Pigs.Animals : an open access journal from MDPI · 2026Article
- Advancing human ovarian biology in tandem with clinical care: considerations for collecting ovarian tissue for research after oophorectomy for tissue cryopreservation.Frontiers in endocrinology · 2026Review
- Fosl2 facilitates chromatin accessibility to determine developmental events during follicular maturation.Nature communications · 2025Article
- Integrative Single-Cell Analysis Reveals Iron Overload-Induced Senescence and Metabolic Reprogramming in Ovarian Endometriosis-Associated Infertility.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Hormonally-regulated KCTD14 Expression Is Higher in Oocytes from Antral Follicles Compared to Those from Preantral Follicles in Mice, with Implications in Fertility.Reproductive sciences (Thousand Oaks, Calif.) · 2025Article
- Intrinsic differences in mTOR activity mediates lineage-specific responses to cyclophosphamide in mouse and human granulosa cells.Journal of ovarian research · 2025Article
- New insights into the ovulatory process in the human ovary.Human reproduction update · 2025Review
- Proteomic Analysis ofAntibiotics (Basel, Switzerland) · 2024Article
- Alpha-Lipoic Acid Ameliorates Impaired Steroidogenesis in Human Granulosa Cells Induced by Advanced Glycation End-Products.Iranian journal of medical sciences · 2024Article
- Combined Analysis of the Transcriptome, Proteome and Metabolome in Human Cryopreserved Sperm.The world journal of men's health · 2024Article
- Primary ovarian insufficiency: update on clinical and genetic findings.Frontiers in endocrinology · 2024Review
- Pyridoxamine protects human granulosa cells against advanced glycation end-products-induced steroidogenesis disturbances.Molecular biology reports · 2023Article
- Equine maternal aging affects the metabolomic profile of oocytes and follicular cells during different maturation time points.Frontiers in cell and developmental biology · 2023Article
- Maturation and culture affect the metabolomic profile of oocytes and follicular cells in young and old mares.Frontiers in cell and developmental biology · 2023Article
- Role of pyroglutamic acid in cumulus cells of women with polycystic ovary syndrome.Journal of assisted reproduction and genetics · 2022Article
- Role of EGFR expressed on the granulosa cells in the pathogenesis of polycystic ovarian syndrome.Frontiers in endocrinology · 2022Article
- Dysregulated sphingolipid metabolism and autophagy in granulosa cells of women with endometriosis.Frontiers in endocrinology · 2022Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This study evaluated the differences in metabolites between cumulus cells (CCs) and mural granulosa cells (MGCs) from human preovulatory follicles to understand the mechanism of oocyte maturation involving CCs and MGCs. CCs and MGCs were collected from women who were undergoing in vitro fertilization (IVF)/intracytoplasmic sperm injection (ICSI) treatment. The differences in morphology were determined by immunofluorescence. The metabolomics of CCs and MGCs was measured by liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) followed by quantitative polymerase chain reaction (qPCR) and western blot analysis to further confirm the genes and proteins involved in oocyte maturation. CCs and MGCs were cultured for 48 h in vitro, and the medium was collected for detection of hormone levels. There were minor morphological differences between CCs and MGCs. LC-MS/MS analysis showed that there were differences in 101 metabolites between CCs and MGCs: 7 metabolites were upregulated in CCs, and 94 metabolites were upregulated in MGCs. The metabolites related to cholesterol transport and estradiol production were enriched in CCs, while metabolites related to antiapoptosis were enriched in MGCs. The expression of genes and proteins involved in cholesterol transport (ABCA1, LDLR, and SCARB1) and estradiol production (SULT2B1 and CYP19A1) was significantly higher in CCs, and the expression of genes and proteins involved in antiapoptosis (CRLS1, LPCAT3, and PLA2G4A) was significantly higher in MGCs. The level of estrogen in CCs was significantly higher than that in MGCs, while the progesterone level showed no significant differences. There are differences between the metabolomes of CCs and MGCs. These differences may be involved in the regulation of oocyte maturation.
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Registered trials
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