ArticleAnimal reproduction2021
Spirulina platensis extract addition to semen extender enhances cryotolerance and fertilizing potentials of buffalo bull spermatozoa.
Article in Animal reproduction, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Effects of Supplementing Semen Extenders With Spirulina platensis Extract on Post-Thaw Ram Sperm Quality.Veterinary medicine and science · 2026Article
- C-phycocyanin protects against obesity-associated type 2 diabetes-induced male fertility dysfunction via key enzyme regulation.Asian journal of andrology · 2026Article
- Antioxidant Activity of Stallion Spermatozoa After Cryopreservation with Natural Antioxidant-Supplemented Extenders.Animals : an open access journal from MDPI · 2026Article
- Effect of nano-spirulina (Spirulina platensis) supplementation to tris egg yolk extender on CASA motility and functional quality of cryopreserved Zaraibi goat sperm.Veterinary research communications · 2026Article
- Holotomography and Multivariate Analysis Reveal Donor-Specific Responses to Antioxidant Supplementation During Stallion Sperm Cryopreservation.Antioxidants (Basel, Switzerland) · 2026Article
- Enhancing Stallion Semen Cryopreservation: Selected Antioxidant Extracts and Sperm Freezability.Antioxidants (Basel, Switzerland) · 2025Article
- The optimal combination of cooling and equilibration durations, along with the addition of melatonin, gamma-oryzanol, and canthaxanthin, for improving swamp buffalo semen cryopreservation quality.Veterinary world · 2024Article
- Effect of autologous platelet-rich plasma on the fertility and quality of cryopreserved buffalo bull semen: a comparative study using OptiXcell® and tris egg yolk extenders.BMC veterinary research · 2024Article
- Glutathione and selenium nanoparticles have a synergistic protective effect during cryopreservation of bull semen.Frontiers in veterinary science · 2023Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The objective of this study was to investigate the effect of Spirulina platensis extract (SPE) addition to the freezing extender on freezability, lipid peroxidation, ultrastructure alterations and fertilizing potentials of frozen-thawed buffalo bull spermatozoa. Semen samples were collected with artificial vagina from five adult fertile bulls and diluted with Tris-base extender containing SPE (1, 5, 10 and 20 μg/mL) or without SPE (control). Diluted semen was cooled to 4 °C throughout one hour and frozen in 0.25 mL straws: prior to being stored in liquid nitrogen. Cryopresreved spermatozoa were assessed for post-thawing sperm motility, viability, acrosomal integrity, ultrastructure changes, antioxidant activities, lipid peroxidation and fertility rate. The current results clearly indicated that adding 10μg/mL SPE to the freezing extender significantly improved (P< 0.05) post-thawing motility and decrease the percentage of acrosomal damage (51.67±6.02% and 16.33±1.46%, respectively) compared with the control (28.33±4.41% and 26.33±1.77%, respectively). Moreover, addition of 10 μg/mL SPE to the semen extender significantly diminished (P< 0.05) MDA concentration (10.66±2.40 nmol/10
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