ArticleNature plants2021
Live single-cell transcriptional dynamics via RNA labelling during the phosphate response in plants.
Article in Nature plants, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- A binding-activated CRISPR/dCas13X imaging system enables precise RNA tracking in plants.PLoS biology · 2026Article
- Quantitative RNA spatial profiling using single-molecule RNA FISH on plant tissue cryosections.Plant communications · 2026Article
- The fluorescence-activating and absorption-shifting tag (FAST), a versatile protein marker for live plant cell imaging.The Plant journal : for cell and molecular biology · 2026Article
- A four-dimensional spatial transcriptome atlas of barley caryopsis development and germination.The Plant cell · 2026Article
- Gene body methylation buffers noise in gene expression in plants.Nucleic acids research · 2026Article
- Root-Specific Signal Modules Mediating Abiotic Stress Tolerance in Fruit Crops.Plants (Basel, Switzerland) · 2026Review
- Coordinated single-nucleus responses for quantitative disease resistance involve a calcium-associated switch in transcriptional noise.Genome biology · 2025Article
- Genome-scale transcriptome augmentation during Arabidopsis thaliana photomorphogenesis.Nature communications · 2025Article
- The chloroplast-targeted long noncoding RNA CHLORELLA mediates chloroplast functional transition across leaf ageing via anterograde signalling.Nature plants · 2025Article
- Live Single-Cell Transcriptional Dynamics in Plant Cells.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Physiological and transcriptional analyses of Arabidopsis primary root growth in response to phosphate starvation under light and dark conditions.Frontiers in plant science · 2025Article
- Sink strength, nutrient allocation, cannabinoid yield, and associated transcript profiles vary in two drug-type Cannabis chemovars.Journal of experimental botany · 2025Article
- Update of phosphate transport regulations.Journal of plant research · 2024Article
- Bet-hedging and variability in plant development: seed germination and beyond.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2024Review
- Comparative functional analyses of PHR1, PHL1, and PHL4 transcription factors in regulating Arabidopsis responses to phosphate starvation.Frontiers in plant science · 2024Article
- smFISH for Plants.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Identification and interest of molecular markers to monitor plant Pi status.BMC plant biology · 2023Article
- Studying root-environment interactions in structured microdevices.Journal of experimental botany · 2023Article
- PHOSPHATE RESPONSE 1 family members act distinctly to regulate transcriptional responses to phosphate starvation.Plant physiology · 2023Article
- CRISPR-dCas13-tracing reveals transcriptional memory and limited mRNA export in developing zebrafish embryos.Genome biology · 2023Article
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14 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plants are constantly adapting to ambient fluctuations through spatial and temporal transcriptional responses. Here, we implemented the latest-generation RNA imaging system and combined it with microfluidics to visualize transcriptional regulation in living Arabidopsis plants. This enabled quantitative measurements of the transcriptional activity of single loci in single cells, in real time and under changing environmental conditions. Using phosphate-responsive genes as a model, we found that active genes displayed high transcription initiation rates (one initiation event every ~3 s) and frequently clustered together in endoreplicated cells. We observed gene bursting and large allelic differences in single cells, revealing that at steady state, intrinsic noise dominated extrinsic variations. Moreover, we established that transcriptional repression triggered in roots by phosphate, a crucial macronutrient limiting plant development, occurred with unexpectedly fast kinetics (on the order of minutes) and striking heterogeneity between neighbouring cells. Access to single-cell RNA polymerase II dynamics in live plants will benefit future studies of signalling processes.
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