Evidence map›Paper›PMID 34373491›Full record

ArticleScientific reports2021

Comparative analysis of freshwater phytoplankton communities in two lakes of Burabay National Park using morphological and molecular approaches.

Dmitry V Malashenkov, Veronika Dashkova, Kymbat Zhakupova, Ivan A Vorobjev, Natasha S Barteneva

Open access · goldAbstract readComparative Study
In one paragraph

Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed, 1 pooled it
3.5field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 1 synthesis or guideline pooled it, 27 citations in OpenAlex.

  1. Systematic Review: Long-Read Sequencing in Algal Studies.International journal of molecular sciences · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Dmitry V MalashenkovNational Laboratory Astana, Nazarbayev University, Nur-Sultan, Kazakhstan. dvmalashenkov@gmail.com.
Veronika DashkovaNational Laboratory Astana, Nazarbayev University, Nur-Sultan, Kazakhstan.
Kymbat ZhakupovaCore Facilities, Nazarbayev University, Nur-Sultan, Kazakhstan.
Ivan A VorobjevNational Laboratory Astana, Nazarbayev University, Nur-Sultan, Kazakhstan. ivan.vorobyev@nu.edu.kz.
Natasha S BartenevaNational Laboratory Astana, Nazarbayev University, Nur-Sultan, Kazakhstan. natalie.barteneva@nu.edu.kz.
Nazarbayev University · KZ

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We analyzed phytoplankton assemblages' variations in oligo-mesotrophic Shchuchie and Burabay lakes using traditional morphological and next-generation sequencing (NGS) approaches. The total phytoplankton biodiversity and abundance estimated by both microscopy and NGS were significantly higher in Lake Burabay than in Lake Shchuchie. NGS of 16S and 18S rRNA amplicons adequately identify phytoplankton taxa only on the genera level, while species composition obtained by microscopic examination was significantly larger. The limitations of NGS analysis could be related to insufficient coverage of freshwater lakes phytoplankton by existing databases, short algal sequences available from current instrumentation, and high homology of chloroplast genes in eukaryotic cells. However, utilization of NGS, together with microscopy allowed us to perform a complete taxonomic characterization of phytoplankton lake communities including picocyanobacteria, often overlooked by traditional microscopy. We demonstrate the high potential of an integrated morphological and molecular approach in understanding the processes of organization in aquatic ecosystem assemblages.

Indexed as

BiodiversityEcosystemFlow CytometryGene LibraryHigh-Throughput Nucleotide SequencingKazakhstanLakesMicroscopy, Phase-ContrastParks, RecreationalPhytoplanktonRNA, Ribosomal, 16SRNA, Ribosomal, 18SRNA, Ribosomal, 16SRNA, Ribosomal, 18S

Identifiers

PMID34373491
PMCPMC8352915
OpenAlexW3188558599

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.