ArticleMicrobial cell factories2021
Screening and evaluation of the strong endogenous promoters in Pichia pastoris.
Article in Microbial cell factories, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Yarrowia lipolytica and Komagataella phaffii (Pichia pastoris) as Hosts for Heterologous Expression of Lipases.Methods in molecular biology (Clifton, N.J.) · 2027Article
- Bacillus subtilis expressing double-stranded RNA induced RNA interference in Tribolium castaneum.Pest management science · 2026Article
- Article
- Enhanced methanol tolerance and utilization in Pichia pastoris revealed by adaptive laboratory evolution.Journal of biological engineering · 2025Article
- Rewiring central metabolism in Komagataella phaffii for efficient mannose synthesis.Microbial cell factories · 2025Article
- Promoter engineering for enhanced 3-hydroxypropionic acid production inSynthetic and systems biotechnology · 2025Article
- Characterizing heterologous protein burden in Komagataella phaffii.FEMS yeast research · 2025Article
- Integrating yeast biodiversity and machine learning for predictive metabolic engineering.FEMS yeast research · 2025Review
- Customizable and stable multilocus chromosomal integration: a novel glucose-dependent selection system in Aureobasidium spp.Biotechnology for biofuels and bioproducts · 2024Article
- Bi-directionalized promoter systems allow methanol-free production of hard-to-express peroxygenases with Komagataella Phaffii.Microbial cell factories · 2024Article
- Promoter engineering enables precise metabolic regulation towards efficient β-elemene production inSynthetic and systems biotechnology · 2024Article
- Effect ofPeerJ · 2024Article
- Exploring and Engineering Novel Strong Promoters for High-Level Protein Expression inMicroorganisms · 2023Article
- Advances in Metabolic Engineering ofJournal of fungi (Basel, Switzerland) · 2023Review
- Functional characterization of the Komagataella phaffii 1033 gene promoter and transcriptional terminator.World journal of microbiology & biotechnology · 2023Article
- Evaluation of reference genes for transcript analyses in Komagataella phaffii (Pichia pastoris).Fungal biology and biotechnology · 2023Article
- Design and construction of microbial cell factories based on systems biology.Synthetic and systems biotechnology · 2023Review
- Isolation and evaluation of strong endogenous promoters for the heterologous expression of proteins in Pichia pastoris.World journal of microbiology & biotechnology · 2022Article
- Marine Microbial-Derived Resource Exploration: Uncovering the Hidden Potential of Marine Carotenoids.Marine drugs · 2022Review
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Authors and funding
5 authors.
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Abstract
backgroundDue to its ability to perform fast and high-density fermentation, Pichia pastoris is not only used as an excellent host for heterologous protein expression but also exhibits good potential for efficient biosynthesis of small-molecule compounds. However, basic research on P. pastoris lags far behind Saccharomyces cerevisiae, resulting in a lack of available biological elements. Especially, fewer strong endogenous promoter elements available for foreign protein expression or construction of biosynthetic pathways were carefully evaluated in P. pastoris. Thus, it will be necessary to identify more available endogenous promoters from P. pastoris.
resultsBased on RNA-seq and LacZ reporter system, eight strong endogenous promoters contributing to higher transcriptional expression levels and β-galactosidase activities in three frequently-used media were screened out. Among them, the transcriptional expression level contributed by P
conclusionsThe novel strong endogenous promoter identified in this study adds to the number of promoter elements available in P. pastoris. And the competitive effect observed here suggests that a careful pre-evaluation is needed when simultaneously and multiply using the same promoter in one yeast strain. This work also provides an effective strategy to identify more novel biological elements for engineering applications in P. pastoris.
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