ArticleBMC genomics2021
Gonadal transcriptomes associated with sex phenotypes provide potential male and female candidate genes of sex determination or early differentiation in Crassostrea gigas, a sequential hermaphrodite mollusc.
Article in BMC genomics, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 50 citations in OpenAlex.
- Deciphering Gamete Differentiation in Ostrea edulis using Laser Capture Microdissection and RNA-seq.Marine biotechnology (New York, N.Y.) · 2026Article
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- Oysters in transition: hermaphrodite oysters display unique DNA methylation patterns in gill tissue.BMC genomics · 2025Article
- Insights from the single-cell level: lineage trajectory and somatic-germline interactions during spermatogenesis in dwarf surfclam Mulinia lateralis.BMC genomics · 2025Article
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- DNA methylation correlates with transcriptional noise in response to elevated pCOEnvironmental epigenetics · 2024Article
- Multi-tissue RNA-Seq Analysis and Long-read-based Genome Assembly Reveal Complex Sex-specific Gene Regulation and Molecular Evolution in the Manila Clam.Genome biology and evolution · 2022Article
- Dynamic transcriptome analysis reveals the gene network of gonadal development from the early history life stages in dwarf surfclam Mulinia lateralis.Biology of sex differences · 2022Article
- Differential DNA methylation in Pacific oyster reproductive tissue in response to ocean acidification.BMC genomics · 2022Article
- Screening of Genes Related to Sex Determination and Differentiation in Mandarin Fish (International journal of molecular sciences · 2022Article
- Transcription Analysis for Core Networks of lncRNAs-mRNAs: Implication for Potential Role in Sterility ofBiology · 2022Article
- Full-Length Transcriptome Sequences Provide Insight Into Hermaphroditism of Freshwater Pearl MusselFrontiers in genetics · 2022Article
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8 authors at 3 institutions in 3 countries.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundIn the animal kingdom, mollusca is an important phylum of the Lophotrochozoa. However, few studies have investigated the molecular cascade of sex determination/early gonadal differentiation within this phylum. The oyster Crassostrea gigas is a sequential irregular hermaphrodite mollusc of economic, physiological and phylogenetic importance. Although some studies identified genes of its sex-determining/-differentiating pathway, this particular topic remains to be further deepened, in particular with regard to the expression patterns. Indeed, these patterns need to cover the entire period of sex lability and have to be associated to future sex phenotypes, usually impossible to establish in this sequential hermaphrodite. This is why we performed a gonadal RNA-Seq analysis of diploid male and female oysters that have not changed sex for 4 years, sampled during the entire time-window of sex determination/early sex differentiation (stages 0 and 3 of the gametogenetic cycle). This individual long-term monitoring gave us the opportunity to explain the molecular expression patterns in the light of the most statistically likely future sex of each oyster.
resultsThe differential gene expression analysis of gonadal transcriptomes revealed that 9723 genes were differentially expressed between gametogenetic stages, and 141 between sexes (98 and 43 genes highly expressed in females and males, respectively). Eighty-four genes were both stage- and sex-specific, 57 of them being highly expressed at the time of sex determination/early sex differentiation. These 4 novel genes including Trophoblast glycoprotein-like, Protein PML-like, Protein singed-like and PREDICTED: paramyosin, while being supported by RT-qPCR, displayed sexually dimorphic gene expression patterns.
conclusionsThis gonadal transcriptome analysis, the first one associated with sex phenotypes in C. gigas, revealed 57 genes highly expressed in stage 0 or 3 of gametogenesis and which could be linked to the future sex of the individuals. While further study will be needed to suggest a role for these factors, some could certainly be original potential actors involved in sex determination/early sex differentiation, like paramyosin and could be used to predict the future sex of oysters.
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