ArticleACS biomaterials science & engineering2021
Dual Functionalization of Gelatin for Orthogonal and Dynamic Hydrogel Cross-Linking.
Article in ACS biomaterials science & engineering, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 37 citations in OpenAlex.
- Injectable Hydrogels for Breast Cancer Therapy: From Tumor Microenvironment-Responsive and Actively Targeted Drug Delivery to Immunotherapy and Theranostics.Pharmaceutics · 2026Review
- Functional Group Chemistry Modulates Cellular Responses to Soluble Gelatin Derivatives Independent of Crosslinking.Biomolecules · 2026Article
- Dynamic granular hydrogels to assess pancreatic cancer cell fate.Biomaterials science · 2026Article
- Heterogeneous polymerization via two-step crosslinking for tunable microribbon hydrogels.Biofabrication · 2025Article
- Merging of Two Photoredox Cycles with One Perovskite Catalyst Achieving Dual Functionalization: N-Heterocyclization and Site-Selective Bromination of N-Arylamines.The Journal of organic chemistry · 2025Article
- Chemically defined and dynamic click hydrogels support hair cell differentiation in human inner ear organoids.Stem cell reports · 2025Article
- Fast-Relaxing Hydrogels Promote Pancreatic Adenocarcinoma Cell Aggressiveness through Integrin β1 Signaling.Biomacromolecules · 2025Article
- Aqueous Synthesis of Poly(ethylene glycol)-amide-Norbornene-Carboxylate for Modular Hydrogel Crosslinking.Advanced materials interfaces · 2025Article
- Photo-responsive decellularized small intestine submucosa hydrogels.Advanced functional materials · 2024Article
- Viscoelastic stiffening of gelatin hydrogels for dynamic culture of pancreatic cancer spheroids.Acta biomaterialia · 2024Article
- Orthogonally Crosslinked Gelatin-Norbornene Hydrogels for Biomedical Applications.Macromolecular bioscience · 2024Review
- Enhanced angiogenesis in porous poly(ε-caprolactone) scaffolds fortified with methacrylated hyaluronic acid hydrogel after subcutaneous transplantation.Biomaterials translational · 2024Article
- Digital Light Processing 3D Bioprinting of Gelatin-Norbornene Hydrogel for Enhanced Vascularization.Macromolecular bioscience · 2023Article
- Dynamic Hydrogels with Viscoelasticity and Tunable Stiffness for the Regulation of Cell Behavior and Fate.Materials (Basel, Switzerland) · 2023Review
- Advances in Gelatin Bioinks to Optimize Bioprinted Cell Functions.Advanced healthcare materials · 2023Review
- Viscoelastic hydrogels for interrogating pancreatic cancer-stromal cell interactions.Materials today. Bio · 2023Article
- Alginate-Gelatin Self-Healing Hydrogel Produced via Static-Dynamic Crosslinking.Molecules (Basel, Switzerland) · 2023Article
- Poly(ethylene glycol)-Norbornene as a Photoclick Bioink for Digital Light Processing 3D Bioprinting.ACS applied materials & interfaces · 2023Article
- Bioinspired Hydrogels as Platforms for Life-Science Applications: Challenges and Opportunities.Polymers · 2022Review
- Construction and osteogenic effects of 3D-printed porous titanium alloy loaded with VEGF/BMP-2 shell-core microspheres in a sustained-release system.Frontiers in bioengineering and biotechnology · 2022Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Gelatin-based hydrogels are widely used in biomedical fields because of their abundance of bioactive motifs that support cell adhesion and matrix remodeling. Although inherently bioactive, unmodified gelatin exhibits temperature-dependent rheology and solubilizes at body temperature, making it unstable for three-dimensional (3D) cell culture. Therefore, the addition of chemically reactive motifs is required to render gelatin-based hydrogels with highly controllable cross-linking kinetics and tunable mechanical properties that are critical for 3D cell culture. This article provides a series of methods toward establishing orthogonally cross-linked gelatin-based hydrogels for dynamic 3D cell culture. In particular, we prepared dually functionalized gelatin macromers amenable for sequential, orthogonal covalent cross-linking. Central to this material platform is the synthesis of norbornene-functionalized gelatin (GelNB), which forms covalently cross-linked hydrogels via orthogonal thiol-norbornene click cross-linking. Using GelNB as the starting material, we further detail the methods for synthesizing gelatin macromers susceptible to hydroxyphenylacetic acid (HPA) dimerization (i.e., GelNB-HPA) and hydrazone bonding (i.e., GelNB-CH) for on-demand matrix stiffening. Finally, we outline the protocol for synthesizing a gelatin macromer capable of adjusting hydrogel stress relaxation via boronate ester bonding (i.e., GelNB-BA). The combination of these orthogonal chemistries affords a wide range of gelatin-based hydrogels as biomimetic matrices in tissue engineering and regenerative medicine applications.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.