ArticleMolecular systems biology2021
Adaptive laboratory evolution of microbial co-cultures for improved metabolite secretion.
Article in Molecular systems biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Reciprocal cross-feeding between bacteria can limit the emergence of metabolic dependencies.Applied and environmental microbiology · 2026Article
- Species sorting shapes the divergence of a traditional fermented dairy-derived bacterial community with repeatable functionality during propagation with alternative substrates.World journal of microbiology & biotechnology · 2026Article
- Adaptive laboratory evolution with ethionine identifies novel genetic determinants for enhanced protein and methionine accumulation in Saccharomyces cerevisiae.Journal of biological engineering · 2026Article
- Microbial biotechnology meets ecological theory: a unified framework to design industrial microbial consortia.International microbiology : the official journal of the Spanish Society for Microbiology · 2026Review
- Cooperativity in microbial biotechnology: synthetic consortia as emerging metabolic engineering strategy for sustainable bioproduction.Frontiers in bioengineering and biotechnology · 2026Review
- Exploring genetic adaptation and microbial dynamics in engineered anaerobic ecosystems via strain-level metagenomics.Cell genomics · 2025Article
- Strengthen or Weaken: Evolutionary Directions of Cross-Feeding After Formation.Environmental microbiology reports · 2025Review
- Sourdough Microbiota for Improving Bread Preservation and Safety: Main Directions and New Strategies.Foods (Basel, Switzerland) · 2025Review
- Industrial Microbial Technologies for Feed Protein Production from Non-Protein Nitrogen.Microorganisms · 2025Review
- Phocaeicola vulgatus shapes the long-term growth dynamics and evolutionary adaptations of Clostridioides difficile.Cell host & microbe · 2025Article
- Strategies and tools to construct stable and efficient artificial coculture systems as biosynthetic platforms for biomass conversion.Biotechnology for biofuels and bioproducts · 2024Review
- A framework for understanding collective microbiome metabolism.Nature microbiology · 2024Review
- Enhanced metabolic entanglement emerges during the evolution of an interkingdom microbial community.Nature communications · 2024Article
- Human gut microbiota interactions shape the long-term growth dynamics and evolutionary adaptations ofbioRxiv : the preprint server for biology · 2024Article
- Metabolic exchanges are ubiquitous in natural microbial communities.Nature microbiology · 2023Review
- Discovery of novel amino acid production traits by evolution of synthetic co-cultures.Microbial cell factories · 2023Article
- Unravelling metabolic cross-feeding in a yeast-bacteria community usingMolecular systems biology · 2023Article
- A Two-Compartment Fermentation System to Quantify Strain-Specific Interactions in Microbial Co-Cultures.Bioengineering (Basel, Switzerland) · 2023Article
- Cyanobacteria as cell factories for the photosynthetic production of sucrose.Frontiers in microbiology · 2023Review
- Adaptive Laboratory Evolution of Microorganisms: Methodology and Application for Bioproduction.Microorganisms · 2022Review
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Adaptive laboratory evolution has proven highly effective for obtaining microorganisms with enhanced capabilities. Yet, this method is inherently restricted to the traits that are positively linked to cell fitness, such as nutrient utilization. Here, we introduce coevolution of obligatory mutualistic communities for improving secretion of fitness-costly metabolites through natural selection. In this strategy, metabolic cross-feeding connects secretion of the target metabolite, despite its cost to the secretor, to the survival and proliferation of the entire community. We thus co-evolved wild-type lactic acid bacteria and engineered auxotrophic Saccharomyces cerevisiae in a synthetic growth medium leading to bacterial isolates with enhanced secretion of two B-group vitamins, viz., riboflavin and folate. The increased production was specific to the targeted vitamin, and evident also in milk, a more complex nutrient environment that naturally contains vitamins. Genomic, proteomic and metabolomic analyses of the evolved lactic acid bacteria, in combination with flux balance analysis, showed altered metabolic regulation towards increased supply of the vitamin precursors. Together, our findings demonstrate how microbial metabolism adapts to mutualistic lifestyle through enhanced metabolite exchange.
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