Evidence map›Paper›PMID 34370208›Full record

ArticleMolecular biology reports2021

Molecular cloning and sequence analysis of a mitogen-activated protein kinase gene in the Antarctic yeast Rhodotorula mucilaginosa AN5.

Cuijuan Shi, Hong Zhang, Kai Yu, Yingying Wang, Jie Jiang, Yun Ju, Chuanzhou Zhang, Ziyi Cheng, Guangfeng Kan

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Article in Molecular biology reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 48% of its field
1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 4 citations in OpenAlex.

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4 · The record

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5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Cuijuan ShiSchool of Marine Science and Technology, Harbin Institute of Technology at Weihai, Weihai, 264209, China.
Hong ZhangSchool of Marine Science and Technology, Harbin Institute of Technology at Weihai, Weihai, 264209, China.
Kai YuSchool of Marine Science and Technology, Harbin Institute of Technology at Weihai, Weihai, 264209, China.
Yingying WangDepartment of Optoelectronic Science, Harbin Institute of Technology at Weihai, Weihai, 264209, People's Republic of China.
Jie JiangSchool of Marine Science and Technology, Harbin Institute of Technology at Weihai, Weihai, 264209, China.
Yun JuSchool of Marine Science and Technology, Harbin Institute of Technology at Weihai, Weihai, 264209, China.
Chuanzhou ZhangSchool of Marine Science and Technology, Harbin Institute of Technology at Weihai, Weihai, 264209, China.
Ziyi ChengSchool of Marine Science and Technology, Harbin Institute of Technology at Weihai, Weihai, 264209, China.
Guangfeng KanSchool of Marine Science and Technology, Harbin Institute of Technology at Weihai, Weihai, 264209, China. gfkan@hit.edu.cn.ORCID http://orcid.org/0000-0002-5357-7204
Harbin Institute of Technology · CN

Funding

Key Technology Research and Development Program of Shandong 2019GHY112047Natural Scientific Research Innovation Foundation in Harbin Institute of Technology HIT.NSRIF.2016085
6 · The paper itself

Abstract

backgroundThe mitogen-activated protein kinase (MAPK) cascades play important roles in various signaling transduction networks of biotic and abiotic stress responses. However, MAPK signaling pathways in cold-active yeast Rhodotorula mucilaginosa have not been reported comprehensively. METHODS AND

resultsIn the present study, MAPK gene (RmMAPK) was first cloned and characterized from Antarctic sea ice yeast R. mucilaginosa AN5. The full length of the RmMAPK gene is 1086 bp and encodes a 361 amino acids protein with a predicted molecular mass of 40.9 kDa and a pI of 5.25. The RmMAPK contains 11 MAPK conserved subdomains and the phosphorylation motif TGY located in the activation loop of the kinase. Quantitative real-time PCR and western blot assay revealed that the expression and phosphorylation level of RmMAPK up-regulated rapidly and significantly when yeast cells were subjected to low temperature (4 °C), high salinity (120‰ NaCl) and heavy metal (2 mmol/L CuCl

conclusionsAll data suggested that the MAPK cascades might act as a key function in response to extreme stresses, such as low temperature, high salinity and heavy metal.

Indexed as

Amino Acid SequenceCloning, MolecularMAP Kinase Signaling SystemMitogen-Activated Protein KinasesPlant ProteinsRhodotorulaSequence Analysis, DNAMitogen-Activated Protein KinasesPlant ProteinsAbiotic stressesAntarctica yeastMitogen-activated protein kinaseSequence characterization

Identifiers

PMID34370208
OpenAlexW3133742376

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.