ArticleDevelopment (Cambridge, England)2021
Live imaging of the Drosophila ovarian niche shows spectrosome and centrosome dynamics during asymmetric germline stem cell division.
Article in Development (Cambridge, England), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 28 citations in OpenAlex.
- Biochemical principles of SMAD signaling across the animal kingdom.The Biochemical journal · 2026Review
- Ecdysone receptor autonomously controls germ cell differentiation in the Drosophila ovary.Development (Cambridge, England) · 2026Article
- Bridges under construction: the dynamics of ring canal expansion during Drosophila oogenesis.Developmental biology · 2026Review
- Natural Genetic Variation Impacts Stress-Induced Quiescence and Regeneration in Response to Rapamycin.Cells · 2026Article
- Article
- Rapamycin Differentially Impacts Germline Stem Cell Quiescence Across Diverse Genetic Backgrounds ofbioRxiv : the preprint server for biology · 2025Article
- PIWI proteins and piRNAs: key regulators of stem cell biology.Frontiers in cell and developmental biology · 2025Review
- Emerin preserves stem cell survival through maintenance of centrosome and nuclear lamina structure.Development (Cambridge, England) · 2024Article
- piRNAs are regulators of metabolic reprogramming in stem cells.Nature communications · 2024Article
- Optimal performance objectives in the highly conserved bone morphogenetic protein signaling pathway.NPJ systems biology and applications · 2024Article
- Niche Tet maintains germline stem cells independently of dioxygenase activity.The EMBO journal · 2024Article
- Article
- Spatial regulation of Drosophila ovarian Follicle Stem Cell division rates and cell cycle transitions.PLoS genetics · 2023Article
- β-importin Tnpo-SR promotes germline stem cell maintenance and oocyte differentiation in female Drosophila.Developmental biology · 2023Article
- Stress-induced reversible cell-cycle arrest requires PRC2/PRC1-mediated control of mitophagy in Drosophila germline stem cells and human iPSCs.Stem cell reports · 2023Article
- Visualizing Fusome Morphology via Tubulin Immunofluorescence in Drosophila Ovarian Germ Cells.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Polarity Events in theFrontiers in cell and developmental biology · 2022Review
- Regulation of Mating-Induced Increase in Female Germline Stem Cells in the Fruit FlyFrontiers in physiology · 2021Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Drosophila female germline stem cells (GSCs) are found inside the cellular niche at the tip of the ovary. They undergo asymmetric divisions to renew the stem cell lineage and to produce sibling cystoblasts that will in turn enter differentiation. GSCs and cystoblasts contain spectrosomes, membranous structures essential for orientation of the mitotic spindle and that, particularly in GSCs, change shape depending on the cell cycle phase. Using live imaging and a fusion protein of GFP and the spectrosome component Par-1, we follow the complete spectrosome cycle throughout GSC division and quantify the relative duration of the different spectrosome shapes. We also determine that the Par-1 kinase shuttles between the spectrosome and the cytoplasm during mitosis and observe the continuous addition of new material to the GSC and cystoblast spectrosomes. Next, we use the Fly-FUCCI tool to define, in live and fixed tissues, that GSCs have a shorter G1 compared with the G2 phase. The observation of centrosomes in dividing GSCs allowed us to determine that centrosomes separate very early in G1, before centriole duplication. Furthermore, we show that the anterior centrosome associates with the spectrosome only during mitosis and that, upon mitotic spindle assembly, it translocates to the cell cortex, where it remains anchored until centrosome separation. Finally, we demonstrate that the asymmetric division of GSCs is not an intrinsic property of these cells, as the spectrosome of GSC-like cells located outside of the niche can divide symmetrically. Thus, GSCs display unique properties during division, a behaviour influenced by the surrounding niche.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.