ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2021
Oligo-FISH barcode in beans: a new chromosome identification system.
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Chromosome painting in plants: history and future perspectives.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2026Review
- Comparative Oligo-FISH Mapping Illuminates Chromosomal Evolution Among Rutaceae Species Diverged Over 50 Million Years.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Complete telomere-to-telomere genomes of cowpea reveal insights into centromere evolution in Phaseoleae.Horticulture research · 2026Article
- Cytogenomic and phylogenomic evidence for new infrageneric relationships in Macroptilium (Benth.) beans.Annals of botany · 2026Article
- Chromosomal variations and evolution in Arachis species revealed by single-copy FISH karyotyping.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Article
- Thriving or Withering? Plant Molecular Cytogenetics in the First Quarter of the 21st Century.International journal of molecular sciences · 2025Review
- "End-to-End Chromosome Fusion" as the Main Driver of Descending Dysploidy inPlants (Basel, Switzerland) · 2025Article
- A cost-effective oligo-based barcode system for chromosome identification in longan and lychee.Horticulture research · 2025Article
- Oligo-FISH barcode chromosome identification system provides novel insights into the natural chromosome aberrations propensity in the autotetraploid cultivated alfalfa.Horticulture research · 2025Article
- Cytomolecular diversity among Vigna Savi (Leguminosae) subgenera.Protoplasma · 2024Article
- Divergence of 10 satellite repeats in Artemisia (Asteraceae: Anthemideae) based on sequential fluorescence in situ hybridization analysis: evidence for species identification and evolution.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2024Article
- Unravelling inversions: Technological advances, challenges, and potential impact on crop breeding.Plant biotechnology journal · 2024Review
- Oligo-barcode illuminates holocentric karyotype evolution inFrontiers in plant science · 2024Article
- Article
- All nonhomologous chromosomes and rearrangements inFrontiers in plant science · 2023Article
- Reconstruction of karyotypic evolution in Saccharum spontaneum species by comparative oligo-FISH mapping.BMC plant biology · 2022Article
- BAC-FISH Based Physical Map of Endangered CatfishInternational journal of molecular sciences · 2022Article
- Five Species ofGenes · 2022Article
- Accurate Chromosome Identification in the Prunus SubgenusInternational journal of molecular sciences · 2022Article
- Five Fabaceae Karyotype and Phylogenetic Relationship Analysis Based on Oligo-FISH for 5S rDNA and (AGGenes · 2022Article
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Authors and funding
10 authors.
Funding
Abstract
key messageAn Oligo-FISH barcode system was developed for two model legumes, allowing the identification of all cowpea and common bean chromosomes in a single FISH experiment, and revealing new chromosome rearrangements. The FISH barcode system emerges as an effective tool to understand the chromosome evolution of economically important legumes and their related species. Current status on plant cytogenetic and cytogenomic research has allowed the selection and design of oligo-specific probes to individually identify each chromosome of the karyotype in a target species. Here, we developed the first chromosome identification system for legumes based on oligo-FISH barcode probes. We selected conserved genomic regions between Vigna unguiculata (Vu, cowpea) and Phaseolus vulgaris (Pv, common bean) (diverged ~ 9.7-15 Mya), using cowpea as a reference, to produce a unique barcode pattern for each species. We combined our oligo-FISH barcode pattern with a set of previously developed FISH probes based on BACs and ribosomal DNA sequences. In addition, we integrated our FISH maps with genome sequence data. Based on this integrated analysis, we confirmed two translocation events (involving chromosomes 1, 5, and 8; and chromosomes 2 and 3) between both species. The application of the oligo-based probes allowed us to demonstrate the participation of chromosome 5 in the translocation complex for the first time. Additionally, we detailed a pericentric inversion on chromosome 4 and identified a new paracentric inversion on chromosome 10. We also detected centromere repositioning associated with chromosomes 2, 3, 5, 7, and 9, confirming previous results for chromosomes 2 and 3. This first barcode system for legumes can be applied for karyotyping other Phaseolinae species, especially non-model, orphan crop species lacking genomic assemblies and cytogenetic maps, expanding our understanding of the chromosome evolution and genome organization of this economically important legume group.
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