Evidence map›Paper›PMID 34367688›Full record

ArticleJournal of parasitology research2021

Optimization of a Loop-Mediated Isothermal Amplification Assay as a Point-of-Care Tool for the Detection of

Kinyatta Nancy, Wambua Lillian, Mutahi Wilkinson, Mugasa Claire, Kamau Luna, Wachira Dorcas, Githae Rosemary, Lusweti Japheth, Ichugu Christine, Waigi Emily and 1 more

Open access · goldAbstract read
In one paragraph

Article in Journal of parasitology research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
0.5field-weighted citation impact, top 34% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 9 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 3 institutions in 2 countries.

Kinyatta NancyKenya Medical Research Institute, Centre for Biotechnology Research and Development, P.O. Box 54840-00200, Nairobi, Kenya.ORCID https://orcid.org/0000-0002-8927-4790
Wambua LillianSchool of Biological Science, University of Nairobi, 30772-00100, Nairobi, Kenya.
Mutahi WilkinsonSchool of Biological Science, University of Nairobi, 30772-00100, Nairobi, Kenya.
Mugasa ClaireCollege of Veterinary Medicine, Animal Resources and Biosecurity, Makerere University Kampala, 7062 Kampala, Uganda.
Kamau LunaKenya Medical Research Institute, Centre for Biotechnology Research and Development, P.O. Box 54840-00200, Nairobi, Kenya.
Wachira DorcasKenya Medical Research Institute, Centre for Biotechnology Research and Development, P.O. Box 54840-00200, Nairobi, Kenya.
Githae RosemaryKenya Medical Research Institute, Centre for Biotechnology Research and Development, P.O. Box 54840-00200, Nairobi, Kenya.
Lusweti JaphethKenya Medical Research Institute, Centre for Biotechnology Research and Development, P.O. Box 54840-00200, Nairobi, Kenya.
Ichugu ChristineKenya Medical Research Institute, Centre for Biotechnology Research and Development, P.O. Box 54840-00200, Nairobi, Kenya.
Waigi EmilyKenya Medical Research Institute, Centre for Biotechnology Research and Development, P.O. Box 54840-00200, Nairobi, Kenya.
Kagai JimKenya Medical Research Institute, Centre for Biotechnology Research and Development, P.O. Box 54840-00200, Nairobi, Kenya.
Kenya Medical Research Institute · KEUniversity of Nairobi · KEMakerere University · UG

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

introductionAccurate detection of filarial parasites in humans and vectors is essential for the implementation and evaluation of Global and National Programs to eliminate lymphatic filariasis. Immunological methods to detect infection are available; however, cross-reactivity issues have been reported in most of them. Nucleic acid-based molecular assays offer high levels of specificity and sensitivity and can be used to detect the infections.

methodsIn this study, we evaluated loop-mediated isothermal amplification (LAMP) tests to amplify

resultsThe sensitivity of the evaluated

conclusionIn this study, LAMP assays coupled with fluorescence dye detection have been found to be suitable for diagnosis and monitoring of

Identifiers

PMID34367688
PMCPMC8337116
OpenAlexW3185251950

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.