ArticlePhysiology and molecular biology of plants : an international journal of functional plant biology2021
Comparative transcriptome between male fertile and male sterile alfalfa (
Article in Physiology and molecular biology of plants : an international journal of functional plant biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed.
- Transcriptomics and metabolomics analyses reveal pollen abortion mechanism in alfalfa early stage male sterile lines.Frontiers in plant science · 2024Article
- Conjunctive Analyses of BSA-Seq and BSR-Seq Unveil theInternational journal of molecular sciences · 2022Article
- Differentially expressed genes related to plant height and yield in two alfalfa cultivars based on RNA-seq.PeerJ · 2022Article
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Authors and funding
6 authors.
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Abstract
Male sterility is an important factor in improving crop quality and yield through heterosis breeding. In this study, we analyzed the transcriptomes of male fertile (MF) and male sterile (MS) alfalfa flower buds using the Illumina HiSeq™ 4000 platform. A total of 54.05 million clean reads were generated and assembled into 65,777 unigenes with an average length of 874 bp. The differentially expressed genes (DEGs) between the MF and MS flowers at three stages of pollen development were identified, and there were 3832, 5678 and 5925 DEGs respectively in stages 1, 2 and 3. GO and KEGG functional enrichment analysis revealed 12, 12, 6 and 12 key branch-point genes involved in circadian rhythm, transcription factors, pollen development and flavonoid biosynthesis. Our findings provide novel insights into the mechanism of male sterility in alfalfa. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s12298-021-01026-x.
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