ArticleThe Science of the total environment2021
Development of a reproducible method for monitoring SARS-CoV-2 in wastewater.
Article in The Science of the total environment, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Assessing RNA integrity by digital RT-PCR: Influence of extraction, storage, and matrices.Biology methods & protocols · 2024Article
- A semi-automated and high-throughput approach for the detection of honey bee viruses in bee samples.PloS one · 2024Article
- Conservation tools: the next generation of engineering-biology collaborations.Journal of the Royal Society, Interface · 2023Review
- Intensity of sample processing methods impacts wastewater SARS-CoV-2 whole genome amplicon sequencing outcomes.The Science of the total environment · 2023Article
- Coupling wastewater-based epidemiological surveillance and modelling of SARS-COV-2/COVID-19: Practical applications at the Public Health Agency of Canada.Canada communicable disease report = Releve des maladies transmissibles au Canada · 2023Article
- A safe haven of SARS-CoV-2 in the environment: Prevalence and potential transmission risks in the effluent, sludge, and biosolids.Geoscience frontiers · 2022Article
- Municipal and neighbourhood level wastewater surveillance and subtyping of an influenza virus outbreak.Scientific reports · 2022Article
- Space-time analysis of COVID-19 cases and SARS-CoV-2 wastewater loading: A geodemographic perspective.Spatial and spatio-temporal epidemiology · 2022Article
- Sewage surveillance for SARS-CoV-2: Molecular detection, quantification, and normalization factors.Current opinion in environmental science & health · 2022Review
- Monitoring COVID-19 through SARS-CoV-2 quantification in wastewater: progress, challenges and prospects.Microbial biotechnology · 2022Review
- Pasteurization, storage conditions and viral concentration methods influence RT-qPCR detection of SARS-CoV-2 RNA in wastewater.The Science of the total environment · 2022Article
- Defining biological and biophysical properties of SARS-CoV-2 genetic material in wastewater.The Science of the total environment · 2022Article
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Authors and funding
7 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Monitoring the genetic signal of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) through RNA titers in wastewater has emerged as a promising strategy for tracking community-scale prevalence of coronavirus disease 2019 (COVID-19). Although many studies of SARS-CoV-2 in wastewater have been conducted around the world, a uniform procedure for concentrating the virus in wastewater is lacking. The goal of this study was to comprehensively evaluate how different methods for concentrating the suspended solids in wastewater affect the associated SARS-CoV-2 RNA signal and the time required for processing samples for wastewater-based epidemiology efforts. We additionally consider the effects of sampling location in the wastewater treatment train (i.e., following preliminary or primary treatment), pasteurization, and RNA extraction method. Comparison of the liquid phase to suspended solids obtained via centrifugation or vacuum filtration suggests that the RNA signal of SARS-CoV-2 preferentially occurs in the solids. Therefore, we assert that the recovery of SARS-CoV-2 from wastewater should focus on suspended solids. Our data indicate that the measured SARS-CoV-2 signal is higher among samples taken from the primary clarifier effluent, as opposed to those taken after preliminary treatment. Additionally, we provide evidence that sample pasteurization at 60 °C for 90 min reduces the SARS-CoV-2 signal by approximately 50-55%. Finally, the results indicate that a magnetic bead approach to RNA extraction leads to a higher SARS-CoV-2 signal than does a silica membrane approach.
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