Evidence map›Paper›PMID 34364133›Full record

ArticleJournal of clinical virology : the official publication of the Pan American Society for Clinical Virology2021

Stability of SARS-CoV-2 IgG in multiple laboratory conditions and blood sample types.

Jamil N Kanji, Ashley Bailey, Jayne Fenton, L Robbin Lindsay, Antonia Dibernardo, Nikki Pl Toledo, Brooks Waitt, Nadine Lecocq, Carla Osiowy, Elizabeth Giles and 5 more

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
0.8field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 17 citations in OpenAlex.

  1. Article
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  8. Preserved SARS-CoV-2 neutralizing IgG activity of in-house manufactured COVID-19 convalescent plasma.Transfusion and apheresis science : official journal of the World Apheresis Association : official journal of the European Society for Haemapheresis · 2023
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 3 institutions in 1 country.

Jamil N KanjiDivision of Infectious Diseases, Department of Medicine, University of Alberta, 8440 - 112 Street NW, Edmonton, AB, T6G 2B7, Canada; Public Health Laboratory, Alberta Precision Laboratories, University of Alberta Hospital, 8440 - 112 Street NW, Edmonton, AB, T6G 2B7, Canada; Department of Laboratory Medicine & Pathology, Faculty of Medicine & Dentistry, University of Alberta, 8440 - 112 Street NW, Edmonton, AB, T6G 2B7, Canada. Electronic address: jamil.kanji@ahs.ca.
Ashley BaileyPublic Health Laboratory, Alberta Precision Laboratories, University of Alberta Hospital, 8440 - 112 Street NW, Edmonton, AB, T6G 2B7, Canada.
Jayne FentonPublic Health Laboratory, Alberta Precision Laboratories, University of Alberta Hospital, 8440 - 112 Street NW, Edmonton, AB, T6G 2B7, Canada.
L Robbin LindsayNational Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street Winnipeg, MB, R3E 3M4.
Antonia DibernardoNational Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street Winnipeg, MB, R3E 3M4.
Nikki Pl ToledoNational Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street Winnipeg, MB, R3E 3M4.
Brooks WaittNational Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street Winnipeg, MB, R3E 3M4.
Nadine LecocqNational Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street Winnipeg, MB, R3E 3M4.
Carla OsiowyNational Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street Winnipeg, MB, R3E 3M4.
Elizabeth GilesNational Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street Winnipeg, MB, R3E 3M4.
Jacqueline DayNational Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street Winnipeg, MB, R3E 3M4.
William StokesDivision of Infectious Diseases, Department of Medicine, University of Alberta, 8440 - 112 Street NW, Edmonton, AB, T6G 2B7, Canada; Public Health Laboratory, Alberta Precision Laboratories, University of Alberta Hospital, 8440 - 112 Street NW, Edmonton, AB, T6G 2B7, Canada; Department of Laboratory Medicine & Pathology, Faculty of Medicine & Dentistry, University of Alberta, 8440 - 112 Street NW, Edmonton, AB, T6G 2B7, Canada.
Clayton MacDonaldPublic Health Laboratory, Alberta Precision Laboratories, University of Alberta Hospital, 8440 - 112 Street NW, Edmonton, AB, T6G 2B7, Canada.
LeeAnn TurnbullPublic Health Laboratory, Alberta Precision Laboratories, University of Alberta Hospital, 8440 - 112 Street NW, Edmonton, AB, T6G 2B7, Canada.
Carmen CharltonPublic Health Laboratory, Alberta Precision Laboratories, University of Alberta Hospital, 8440 - 112 Street NW, Edmonton, AB, T6G 2B7, Canada; Department of Laboratory Medicine & Pathology, Faculty of Medicine & Dentistry, University of Alberta, 8440 - 112 Street NW, Edmonton, AB, T6G 2B7, Canada; Li Ka Shing Institute of Virology, University of Alberta, 6-010 Katz Group Centre for Pharmacy and Health Research, Edmonton, Alberta T6G 2E1, Canada.
Public Health Agency of Canada · CAAlberta Hospital Edmonton · CAUniversity of Alberta · CA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundCOVID-19 seroprevalence studies use serum/plasma samples to detect SARS-CoV-2 IgG. Data supporting alternate specimen types and freeze-thaw antibody stability is lacking. The stability of IgG and other immunoglobulins in multiple blood sample types stored in differing conditions and multiple freeze-thaw cycles (FTCs) was evaluated. MATERIALS AND

methodsSerum, plasma, and heparinized-plasma samples were collected from COVID-19 recovered individuals. Samples underwent testing for SARS-CoV-2 antibodies upon collection, after each of 10-12 FTCs, and storage at -70°C, -20°C, 4°C, and room-temperature for 10-12 days using four high-throughput commercial assays, two rapid-test cassettes, a manual ELISA, and a surrogate neutralization assay.

resultsAll three specimen types were collected from 34 COVID-19 recovered seropositive individuals (≥21 days post-symptoms). Using the Architect and Liaison assays, a positive qualitative SARS-CoV-2 IgG result was detected daily up to 12 FTCs and up to 10 days of storage at different temperatures. An additional 25 plasma samples consistently demonstrated detection of SARS-CoV-2 antibodies daily after 12 FTCs and storage at -20°C using two rapid test cassette assays (SD Biosensor and Hangzhou All Test), manual (Beijing Wantai) and surrogate neutralization (GenScript) ELISAs, and two high-throughput assays (Roche Elecsys nucleocapsid and spike). IgM antibodies were less frequently detected by one of the rapid test cassette assays.

conclusionsSerum, plasma, and heparinized-plasma constitute reliable samples for SARS-CoV-2 antibody detection. In particular, the IgG response was stable and reliably detected after multiple FTCs and storage at common laboratory conditions. IgM detection was variable due to the labile nature of this antibody class.

Indexed as

COVID-19SARS-CoV-2HumansImmunoglobulin GLaboratoriesSensitivity and SpecificitySeroepidemiologic StudiesImmunoglobulin GFreeze-thaw cycleLithium heparinPlasmaSARS-CoV-2 IgGSerumStability

Identifiers

PMID34364133
PMCPMC8310571
OpenAlexW3186062866

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.