ArticlePsychoneuroendocrinology2021
Specificity of plasma oxytocin immunoassays: A comparison of commercial assays and sample preparation techniques using oxytocin knockout and wildtype mice.
Article in Psychoneuroendocrinology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 28 citations in OpenAlex.
- Article
- Article
- Neurophysin-I dynamics upon different pituitary provocation tests in healthy participants.Endocrine connections · 2026Article
- Oxytocin: a neglected hormone in pituitary disease - From function to the diagnosis of a deficiency, resulting clinical relevance, and potential treatment options in endocrinology.Archives of endocrinology and metabolism · 2025Review
- Urinary oxytocin secretion after pituitary surgery, early arginine vasopressin deficiency and syndrome of inappropriate antidiuresis.Endocrine · 2025Article
- Human placental lactogen (human chorionic somatomammotropin) and oxytocin during pregnancy: Individual patterns and associations with maternal-fetal attachment, anxiety, and depression.Hormones and behavior · 2024Article
- Neurophysin I is an analytically robust surrogate biomarker for oxytocin.Psychoneuroendocrinology · 2024Article
- Changes in peripheral oxytocin and vasopressin during a silent month-long Insight meditation retreat.Frontiers in endocrinology · 2024Article
- Changes in salivary oxytocin in response to biologically-relevant events in farm animals: method optimization and usefulness as a biomarker.Frontiers in physiology · 2024Article
- Inaccuracies in plasma oxytocin extraction and enzyme immunoassay techniques.Comprehensive psychoneuroendocrinology · 2023Article
- Close encounters with oxytocin.Comprehensive psychoneuroendocrinology · 2023Review
- Oxytocin measurements in saliva: an analytical perspective.BMC veterinary research · 2023Review
- Measuring oxytocin release in response to gavage: Computational modelling and assay validation.Journal of neuroendocrinology · 2023Article
- Advances in human oxytocin measurement: challenges and proposed solutions.Molecular psychiatry · 2023Review
- Sex, love and oxytocin: Two metaphors and a molecule.Neuroscience and biobehavioral reviews · 2022Review
- What are oxytocin assays measuring? Epitope mapping, metabolites, and comparisons of wildtype & knockout mouse urine.Psychoneuroendocrinology · 2022Article
- Oxytocin-a social peptide? Deconstructing the evidence.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2022Review
- Oxytocin and oxygen: the evolution of a solution to the 'stress of life'.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2022Review
- Endogenous oxytocin, cortisol, and testosterone in response to group singing.Hormones and behavior · 2022Article
- Oxytocin and love: Myths, metaphors and mysteries.Comprehensive psychoneuroendocrinology · 2022Review
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
Oxytocin has garnered much interest due to its role in affective states, social behaviors, and diverse physiological functions. However, approaches for measuring endogenous oxytocin concentrations have generated considerable controversy and debate. Common procedures for measuring oxytocin often produce uncorrelated results, and the detected concentrations frequently vary across two orders of magnitude. These findings have led some researchers to argue that immunoassays of plasma oxytocin may be unreliable and nonspecific, particularly when samples are not first processed using an extraction procedure. Here, we assess the specificity of oxytocin immunoassays using plasma samples from wildtype (WT) and oxytocin knockout (KO) mice. Plasma samples from both genotypes were measured using immunoassay and were measured with or without a solid-phase extraction. Using a commercially available kit from Arbor Assays, we demonstrate that both techniques generate a clear contrast between genotypes, with wildtype samples containing high concentrations of oxytocin (unextracted mean = 468 pg/ml; extracted mean = 381 pg/ml), while knockout samples measured below the lower limit of detection. Analytical validations demonstrated good parallelism and spike recovery for both methods. Furthermore, the same wildtype samples measured with both procedures were highly correlated (r = 0.95), although unextracted samples measured at significantly higher concentrations (p = 2.0 ×10
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.