ArticlePest management science2021
A LAMP (loop-mediated isothermal amplification) test for rapid identification of Khapra beetle (Trogoderma granarium).
Article in Pest management science, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 29 citations in OpenAlex.
- Rapid Point-of-Care Detection ofAnimals : an open access journal from MDPI · 2026Article
- Loop-mediated isothermal amplification (LAMP) assay for identification of Australian Plague Locust (APL), Chortoicetes terminifera (Walker, 1870).Scientific reports · 2026Article
- A new molecular tool for detection of the highly invasive gecko, Hemidactylus frenatus.PloS one · 2026Article
- The relationship between taxonomic classification and applied entomology: stored product pests as a model group.Journal of insect science (Online) · 2025Review
- Rapid on-site differentiation of two invasive whitefly cryptic species using LAMP.Scientific reports · 2025Article
- LAMP Reaction in Plant Disease Surveillance: Applications, Challenges, and Future Perspectives.Life (Basel, Switzerland) · 2024Review
- Two independent LAMP assays for rapid identification of the serpentine leafminer, Liriomyza huidobrensis (Blanchard, 1926) (Diptera: Agromyzidae) in Australia.Scientific reports · 2023Article
- High-quality DNA isolation protocol for detection of Khapra beetle (Dermestidae: Trogoderma granarium Everts, 1898) in standard wheat germ trap.Molecular biology reports · 2023Article
- LAMP (Loop-mediated isothermal amplification) assay for rapid identification of Varroa mites.Scientific reports · 2023Article
- LAMP assay for the detection of the Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Psylloidea: Psyllidae).Scientific reports · 2023Article
- Taxonomy and Biological Control: New Challenges in an Old Relationship.Neotropical entomology · 2023Article
- Development of an array of molecular tools for the identification of khapra beetle (Trogoderma granarium), a destructive beetle of stored food products.Scientific reports · 2023Article
- Development of a Duplex LAMP Assay with Probe-Based Readout for Simultaneous Real-Time Detection ofInternational journal of molecular sciences · 2023Article
- Article
- Loop-mediated isothermal amplification (LAMP) assays for detection of the New Guinea fruit fly Bactrocera trivialis (Drew) (Diptera: Tephritidae).Scientific reports · 2022Article
- A LAMP Assay for the Detection ofInsects · 2022Article
- Investigation and validation of labelling loop mediated isothermal amplification (LAMP) products with different nucleotide modifications for various downstream analysis.Scientific reports · 2022Article
- A diagnostic LAMP assay for rapid identification of an invasive plant pest, fall armyworm Spodoptera frugiperda (Lepidoptera: Noctuidae).Scientific reports · 2022Article
- Detection of Khapra Beetle Environmental DNA Using Portable Technologies in Australian Biosecurity.Frontiers in insect science · 2022Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundKhapra beetle (Trogoderma granarium Everts) is a significant pest of food products around the world, causing great losses of stored grain and produce, with export restrictions imposed on countries with established beetle populations. Khapra beetle is a high-priority exotic invertebrate pest in many countries requiring a rapid quarantine/biosecurity response when incursions occur. To address this, we developed a novel Khapra LAMP (loop-mediated isothermal amplification) assay using a portable real-time fluorometer and an additional 18S ribosomal DNA (18S) insect control LAMP assay for confirmation of the presence of insect DNA. Both LAMP tests can be performed either in a portable real-time fluorometer or using simple, visual colorimetric technique.
resultsBoth the Khapra and 18S LAMP tests amplify positive samples within ≤ 25 min, with an anneal derivative temperature of 77.7 ± 0.7 °C for Khapra LAMP test and 88.0 ± 1.0 °C for 18S. The new Khapra LAMP assay is sensitive to very low levels of DNA (1.02 × 10
conclusionThe novel Khapra and 18S LAMP assays should improve speed, accuracy and confidence of detection of Khapra beetle at incursion points and aid rapid biosecurity responses in any country affected, especially as the assays described here are portable and easy to implement in the field conditions where resources are limited. © 2021 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
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