ArticleNature communications2021
The intervening domain is required for DNA-binding and functional identity of plant MADS transcription factors.
Article in Nature communications, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
42 citing papers in PubMed, 84 citations in OpenAlex.
- Integrated Genomic and Functional Analyses IdentifyInternational journal of molecular sciences · 2026Article
- The phytoplasma effector, phyllogen, structurally mimics host plant MADS transcription factors.The Journal of biological chemistry · 2026Article
- The other giant: functional significance and divergence of Type I MADS-box genes in the evolution of land plants.Journal of experimental botany · 2026Review
- No Evidence Supports the Presence of SRF-type MADS-Box Genes in Land Plants.Genome biology and evolution · 2026Article
- Review
- Roles of MADS-box transcription factors in plant responses to abiotic and biotic stresses.Plant communications · 2026Review
- Genome-Wide Analysis of the MADS-Box Gene Family and Expression Pattern Under Abiotic Stresses inInternational journal of molecular sciences · 2026Article
- The PbrMADS1-PbrMYB169 complex has uniquely emerged to regulate lignification of stone cells in pear.Journal of integrative plant biology · 2026Article
- Ectopic Over-Expression ofPlants (Basel, Switzerland) · 2025Article
- The specificity landscape of WRKY transcription factors reveals the bidirectional influence of non-CG methylation.Nucleic acids research · 2025Article
- Two Transcriptional Factors Antagonistically Fine-Tune MIR1863a to Balance Resistance and Yield Traits in Rice.Plant biotechnology journal · 2025Article
- Research Progress on the Regulation of Plant Floral Organ Development by theInternational journal of molecular sciences · 2025Review
- A Novel OsMPK6-OsMADS47-PPKL1/3 Module Controls Grain Shape and Yield in Rice.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Genome-Wide Identification and Expression Analyses of the MADS-Box Gene During Flowering inPlants (Basel, Switzerland) · 2025Article
- Genome-Wide Identification, Characterization, and Expression Analysis of the NAC Transcription Factor Family in Sweet Cherry (Plants (Basel, Switzerland) · 2025Article
- B-class gene GLOBOSA - a facilitator for enriched species diversity of Salvia in the New World?Plant biology (Stuttgart, Germany) · 2025Article
- Article
- Identification of Schizophrenia-Risk Regulatory Variant rs1399178 in the Non-coding Region With Its Impact on NRF1 Binding.CNS neuroscience & therapeutics · 2025Article
- Identification, expression analyses ofPhysiology and molecular biology of plants : an international journal of functional plant biology · 2025Article
- Genome-Wide Investigation ofInternational journal of molecular sciences · 2025Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
15 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The MADS transcription factors (TF) are an ancient eukaryotic protein family. In plants, the family is divided into two main lineages. Here, we demonstrate that DNA binding in both lineages absolutely requires a short amino acid sequence C-terminal to the MADS domain (M domain) called the Intervening domain (I domain) that was previously defined only in type II lineage MADS. Structural elucidation of the MI domains from the floral regulator, SEPALLATA3 (SEP3), shows a conserved fold with the I domain acting to stabilise the M domain. Using the floral organ identity MADS TFs, SEP3, APETALA1 (AP1) and AGAMOUS (AG), domain swapping demonstrate that the I domain alters genome-wide DNA-binding specificity and dimerisation specificity. Introducing AG carrying the I domain of AP1 in the Arabidopsis ap1 mutant resulted in strong complementation and restoration of first and second whorl organs. Taken together, these data demonstrate that the I domain acts as an integral part of the DNA-binding domain and significantly contributes to the functional identity of the MADS TF.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.