ArticleBMC genomics2021
Genome-wide identification of the MADS-box transcription factor family in autotetraploid cultivated alfalfa (Medicago sativa L.) and expression analysis under abiotic stress.
Article in BMC genomics, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
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57 citing papers in PubMed, 111 citations in OpenAlex.
- Identification and characterization of salt-, drought-, and combined salt-drought stress-responsivePlant signaling & behavior · 2026Article
- Genome-wide characterization and expression analysis of the B3 superfamily in response to abiotic stresses in alfalfa (Medicago sativa L.).BMC plant biology · 2026Article
- Comparative transcriptome analysis of sesame flower organs and potential functional analysis of the MIKC-MADS gene family.BMC genomics · 2026Article
- Characterization of the BPC genes in alfalfa and functional verification of MsBPC10 in salt tolerance.BMC plant biology · 2026Article
- Genome-Wide Analysis of the MADS-Box Gene Family and Expression Pattern Under Abiotic Stresses inInternational journal of molecular sciences · 2026Article
- Identification, characterization, and expression profiling of genes encoding tubby-like proteins in rice exposed to realistic environmental contamination of anilofos and bentazone.Journal, genetic engineering & biotechnology · 2026Article
- Integrated ion, hormone, and proteomic analyses reveal the mechanisms of drought resistance in alfalfa (Medicago sativa).BMC plant biology · 2026Article
- Integrated Phylogenomic and Expression Analyses Reveal Lineage-Specific Loss of the Mβ Subfamily and Regulatory Diversification of MADS-Box Genes in Pepper.Plants (Basel, Switzerland) · 2026Article
- Genome-wide identification and expression analysis of the GRAS transcription factor family and its expression profiles in Elymus sibiricus.BMC genomics · 2026Article
- MADS-box gene expression patterns correlate with drought and salt stress responses in Kentucky bluegrass (PeerJ · 2026Article
- Genome-wide identification and characterization ofFrontiers in plant science · 2026Article
- Chromosome-level genome assembly of Hamlin sweet orange and transcriptomic insights into MADS-box gene regulation of secondary fruit development.Frontiers in plant science · 2026Article
- Transcription-metabolism association analysis of molecular mechanisms in sweet orange plants in response to boron deficiency.BMC genomics · 2025Article
- Genome-Wide Identification and Functional Divergence of the Chloride Channel (CLC) Gene Family in Autotetraploid Alfalfa (International journal of molecular sciences · 2025Article
- Genome-wide identification and analysis of the abiotic stress responsiveness of the heat shock protein 90 gene family in Medicago sativa L.BMC plant biology · 2025Article
- Genome-wide characterization of NHX gene family in Medicago sativa under abiotic stress.BMC plant biology · 2025Article
- Genome-wide identification and expression analysis of the GARP superfamily in response to abiotic stresses in alfalfa (Medicago sativa L.).BMC plant biology · 2025Article
- Genome-wide identification and expression pattern analysis of the CPP transcription factor family in Medicago sativa L.BMC plant biology · 2025Article
- Natural Selection as the Primary Driver of Codon Usage Bias in the Mitochondrial Genomes of ThreeGenes · 2025Article
- Genome-Wide Reidentification and Expression Analysis ofInternational journal of molecular sciences · 2025Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundAlfalfa, the "queen of forage", is the most extensively cultivated forage legume in the world. The development and yield of alfalfa are seriously limited by abiotic stress. MADS-box transcription factors are one of the largest gene families and play a pivotal role in plant development and abiotic stress. However, little is known regarding the MADS-box transcription factors in autotetraploid cultivated alfalfa.
resultsIn the present study, we identified 120 MsMADS-box genes in the alfalfa genome. Phylogenetic analysis indicated that 75 type-I MsMADS-box genes were classified into the Mα, Mβ, and Mγ subgroups, and 45 type-II MsMADS-box genes were classified into 11 subgroups. The promoter region of MsMADS-box genes containing several hormone and stress related elements. Chromosomal location analysis revealed that 117 MsMADS-box genes were unevenly distributed on 32 chromosomes, and the remaining three genes were located on unmapped scaffolds. A total of nine pairs of segmental duplications and four groups of tandem duplications were found. Expression analysis showed that MsMADS-box genes were differentially expressed in various tissues and under abiotic stresses. qRT-PCR analysis revealed that the expression profiles of eight selected MsMADS-box genes were distinct under various stresses.
conclusionsIn this study, MsMADS-box genes were identified in the cultivated alfalfa genome based on autotetraploid level, and further confirmed by Gene Ontology (GO) analysis, phylogenetic analysis, sequence features and expression analysis. Taken together, these findings will provide clues for further study of MsMADS-box functions and alfalfa molecular breeding. Our study is the first to systematically identify and characterize the MADS-box transcription factors in autotetraploid cultivated alfalfa (Medicago sativa L.), and eight MsMADS-box genes were significantly involved in response to various stresses.
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