Evidence map›Paper›PMID 34361800›Full record

ReviewMolecules (Basel, Switzerland)2021

An Evolving Technology That Integrates Classical Methods with Continuous Technological Developments: Thin-Layer Chromatography Bioautography.

Meng Wang, Yirong Zhang, Ruijie Wang, Zhibin Wang, Bingyou Yang, Haixue Kuang

Abstract readReview
In one paragraph

Review in Molecules (Basel, Switzerland), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.

0numbers the graph read from it
0cells of the map it votes in
23citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

23 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
  4. Article
  5. Review
  6. Article
  7. Article
  8. Antimicrobial Compounds FromInternational journal of microbiology · 2025
    Article
  9. Article
  10. Article
  11. Article
  12. Actinomycins from Soil-InhabitingMolecules (Basel, Switzerland) · 2023
    Article
  13. Evaluation of antibacterial efficacy of garlic (Journal of advanced veterinary and animal research · 2023
    Article
  14. Article
  15. Review
  16. Frontiers in microbiology · 2023
    Article
  17. Overview on Strategies and Assays for Antibiotic Discovery.Pharmaceuticals (Basel, Switzerland) · 2022
    Review
  18. Article
  19. Review
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Meng WangKey Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, Harbin 150000, China.
Yirong ZhangKey Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, Harbin 150000, China.
Ruijie WangKey Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, Harbin 150000, China.
Zhibin WangKey Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, Harbin 150000, China.
Bingyou YangKey Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, Harbin 150000, China.
Haixue KuangKey Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, Harbin 150000, China.

Funding

Cooperative Scientific Research Project of 'Chunhui Plan' of Ministry of Education HLJ2019035National Natural Science Foundation of China 81803686Special Fund Project of Postdoctor in Heilongjiang Province LBH-Q20180Supporting Project of National Natural Science Foundation of China 2018PP01University Nursing Program for Young Scholars with Creative Talents in Heilongjiang Province UNPYSCT-2020225
6 · The paper itself

Abstract

Thin-layer chromatography (TLC) bioautography is an evolving technology that integrates the separation and analysis technology of TLC with biological activity detection technology, which has shown a steep rise in popularity over the past few decades. It connects TLC with convenient, economic and intuitive features and bioautography with high levels of sensitivity and specificity. In this study, we discuss the research progress of TLC bioautography and then establish a definite timeline to introduce it. This review summarizes known TLC bioautography types and practical applications for determining antibacterial, antifungal, antitumor and antioxidant compounds and for inhibiting glucosidase, pancreatic lipase, tyrosinase and cholinesterase activity constitutes. Nowadays, especially during the COVID-19 pandemic, it is important to identify original, natural products with anti-COVID potential compounds from Chinese traditional medicine and natural medicinal plants. We also give an account of detection techniques, including in situ and ex situ techniques; even in situ ion sources represent a major reform. Considering the current technical innovations, we propose that the technology will make more progress in TLC plates with higher separation and detection technology with a more portable and extensive scope of application. We believe this technology will be diffusely applied in medicine, biology, agriculture, animal husbandry, garden forestry, environmental management and other fields in the future.

Indexed as

AnimalsAnti-Infective AgentsAntineoplastic AgentsAntioxidantsChromatography, Thin LayerDrug DiscoveryEnzyme InhibitorsHumansLuminescent MeasurementsMicrobial Sensitivity TestsPlant ExtractsPlants, MedicinalSensitivity and SpecificityAnti-Infective AgentsAntineoplastic AgentsAntioxidantsEnzyme InhibitorsPlant Extractsactivity detectionbioautographypractical applicationsegregation analysisthin-layer chromatography

Identifiers

PMID34361800
PMCPMC8347725

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.